Molecular Identification And Population Genetic Structure Of Anopheles Dirus Complex (Diptera: Culicidae) From China

Objective: To clarify the taxonomic status on Anopheles dirus complex from China. To study on the molecular population genetic structure, based on the level of complex identification,Methods: Field specimen of An. dirus s. s. identified by morphology were distinguished by diagnostic PCR assay. rDNA-ITS2 and 28S-D3 regions were amplified and sequenced. The genetic diversity of population was analyzed by mtDNA-COI gene and microsatellite DNA polymorphism.Results:1. The sequence differences of rDNA-ITS2 and 28S-D3 and products of diagnostic PCR assay suggested that it was mistakes of member record in An. dirus complex from Yunnan. It should be corrected as: "An. baimaii",An. sp, which need to be nomenclature in future.2. There were 3 and 6 haplotypes in An. dirus and "An. baimaii" based on mtDNA-COI sequence variances. The level of nucleicacid differences in Mengla population was higher than Jiangcheng and Hainan. There were many mutations at low frequency of transduction. The population was all during expanding stage. The gene flow was limited among populations (Fst=0.7999) . The intra-population variation (20.01%) was lower than inter-population (79.99%).3. The average gene diversity was the highest in Mengla population (0.4469 ± 0.2485) by microsatellite DNA polymorphism. There were 14 microsatellite loci which deviated from Hardy-Wenberg equilibrium. The polymorphism of microsatellite loci intra-population was greater than inter-populations and inter-species.Conclusions:l.An. dirus complex from China is complicated. There are at least 4 members by now.2. The difference was low between An. dirus and "An. baimaii". The genetic divergence was appeared significantly in Mengla population of "An. baimaii".