The Antibody Against CART Antagonized The Conditioned Place Preference Induced By Morphine In Mice

CART (Cocaine and Amphetamine Regulated Transcript) peptides are neurotransmitters that received much attention due to their effects on modulating psychostimulant drug actions. To Study the effects of CART peptides on the morphine dependence, the mice conditional place preference (CPP) model was used to test the effects of CART peptides antibody on the CPP induced by morphine in mice.The results showed morphine induced CPP in mice is inhibited significantly by the injection of CART55-102 peptides antibody (4mg/kg) i.v. two hours before daily morphine training. However, the 2mg/kg of CART_55-102 peptide antibody was not inhibited significantly the CPP induced by morphine. Three different concentration (2mg/kg, 4mg/kg, 8mg/kg) of CART_55-102peptide antibody were given after morphine training. The CART_55-102 peptide antibody(4mg/kg, 8mg/kg) inhibited morphine induced CPP (p<0.01), while the 2mg/kg of CART_55-102 peptide antibody did not inhibit morphine induced CPP. Then Three different concentration (2mg/kg, 4mg/kg, 8mg/kg) of CART_55-102 peptide antibody were giventhree days successively after morphine training. We discovered that all concentrations (2mg/kg, 4mg/kg, 8mg/kg) could modify significantly the CPP induced by morphine, moreover the CART55_io2 peptide antibody(4mg/kg, 8mg/kg) were modified thoroughly the CPP induced by morphine(p<0.01).