| Objective: To observe the gene expression profiles in the hippocampus of the rats after chronic cerebral ischemia which can induce progressive or permanent cognitive impairment.Methods: Chronic cerebral ischemia was induced by a permanent, bilateral occlusion of the common carotid arteries (2-VO) of adult rats. Twelve male rats were divided randomly into two groups:six in the control group, six in the operative group. Then the rats were sacrificied two months after the operation, the gene expression profiles of two groups in hippocampus were detected with DNA microarray analysis technique. Semiquantitative RT-PCR was used to verify the expression of selected gene, N-methyl D-aspartate receptor 1.Results: Among the whole 1322 genes contained in the RN-U34 microarray, 16 genes were upregulated, while 28 genes were downregulated in the operated group compared with the control group. The mRNA level of NMDAR1 of the operated groups was significantly decreased compared with that of control group.Conclusion: Chronic cerebral ischemia caused some genes expression changes of hippocampus, among which were several related with cognition, and NMDAR1 expression of hippocampus may contribute to vascular cognitive impairment. |
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