Establishment Of Model For Allogenetic Renal Transplantation In The Rats And The Posttransplant Monitoring Of IL-2, IL-4, IFN-γ In The Plasma Of Grafts.

Objectives: To establish the first rat model of renal allograft transplantation using microsurgical technique in XinJiang, providing a more practical and reliable model for experimental and clinical studies of renal transplantation.On the basis of this model,to observe the levels of serum interleukin-2(IL-2), interleukin (IL-4), interferon-γ(IFN-γ) and their importance in allografts. Methods: First, the model for allogenetic renal transplantation in the rats was established.The healthy adult Wistar and SD rats were adopted as donor and recipient. The left kidneys of the donor rats were removed followed by immediate reperfusion with 4 degrees Celsius H-CA solution, before they were transplanted into the recipient rats with their left kidneys excised and end-to-side anastomosis of the renal arteries and veins and ureter respectively performed end-to-end procedure with microsurgical technique. Then, the normal group, the pseudo operative group, the group of transplantation of allogenetic and congenetic rats were set up. The serum IL-2, IL-4, IFN-γ, serum creatinine (Cr) and BUN were detected on 3rd, 5th, 7th day after renal transplantation, and meanwhile the pathological change on transplanted kidneys were evaluated. Results: The average total time of operation was 108± 15min, a successful rate of 83.3% was achieved inthe production of the experimental modal. The levels of IL-2,IFN-y significantly increased in acute allograft rejection in early stage, and decreased slowly afterwards. No significantly increased of IL-4 occurred in contrast with the control(P>0. 05).There was a positive correlation between levels of Cr, BUN and seriousness of pathological harm on the transplanted kidney, though no significant result occurred to IL-2 and IFN-y.Conclusion: This rats model of renal allograft transplantation has markedly fewer complications with improved survival rate, thus providing a more practical and reliable model for experimental and clinical studies of renal transplantation. Monitoring the serum IL-2, IFN-y might insist in diagnosis of acute allograft rejection in early stage, However can not show the seriousness of pathological harm of acute rejection. The change of levels of IL-4 might not insist in diagnosis of acute rejection.