Osteogenic Growth Peptide Promoted Differentiation From Mesenchymal Stem Cells To Adipocytes Or Osteoblasts

Objective To value the effect of osteogenic growth peptide on the proliferation and differentiation of mesenchymal stem cells in rats.Method Percoll protocol is employed to extract mesenchymal stem cells in rats. The cells were purified through passages and frequent replace of medium. In order to obtain the most efficient concentration of OGP, passage 2 cells were detected by MTT analysis. Passage 3 cells were planted in 6 cell plate. The cells were divided into osteoblasts-induced group and adipocytes- induced group .The medium contained OPG(10^-11 mol/L) .After had been treating 7,14 , 21days, the cell of adipocytes-induced group were identified by detecting total TG respectively. After had been treating 8, 12, 16 days, the cells of osteoblasts-induced group were identified by ALP activity analyses and detecting total Ca. After had been treating 21 days, the cells of adipocytes-induced group were stained by oil red O;Then calcium deposition, after had been treating 30 days , were detected by Von Kossa staining in the cell of osteoblasts-induced group.Results The mesenchymal stem cells, after passage 3, had been induced by adipogenic inducer during 7 days, 14 days or 21days respectively. The percents of adipocytes and the accumulation of triglyceride, compared with negative control, step up significantly. Then, the cells had been induced by osteogenic inducer during 8 days,12 days or 16days respectively. Alkaline phosphatase activities and the accumulation of calcium in induced cells also raises greatly compared with negative control. The calcified nodules in induced cells increases in contrast with that in negative control.Conclusion Osteogenic growth peptide can promote the transfer from mesenchymal stem cells to adipocytes or osteoblasts under different condition.