The Associative Study Of Intercellular Adhesion Molecule 1 And It's Gene Polymorphism In Graves Disease

Objective To investigate the association between Intercellular adhesion moleculel (ICAM-1 ) with the polymorphism of it's the forth exon G241R(721G? A) and the sixth exon K469E ( 1405A? G) and the Graves disease of Chinese Hans in northern China.Methods The serum ICAM-1 concentration and the allele of its the forth exon G241R(721G? A) and the sixth exon K469E ( 1405A? G) were determined by enzyme linked immunosorbent assay(ELISA) and the PCR sequence-specific primers method ( PCR-SSP) in 202 Chinese Hans in northern China, including 139 patients of Graves Disease(GD).According to the age when GD occured, the GD cases were divided into two groups, the early-onset group including 78 cases (<40 years old) and the late-onset group including 61 cases (=40 years old) .The health control group included 63 persons. The statistic analysis was performed by SPSS for 11.0.Comparison of the sequences of allele in every group was performed by Analysis of X~2 test, the sICAM-1 concentration in GD patients with different gene type and other biochemical conclusions were expressed with X±S, T test was applied. A two-tailed P value<0.05 was taken as statistically significant.Results: (1) The sICAM-1 of GD group was higher than in healthy group;(2) The polymorphic site of the forth exon 721 had G and A alleles. ( 3 ) The polymorphic site of the sixth exon 1405 had A and G alleles. (4) The sequence of A allele in the forth exon 721G? A in early GD group was higher the late GD group and the healthy group. ( 5 ) There were no significant differences in the two alleles in the sixth exon 1405A? G among three groups. (6) There were no relationship between the concentration of sICAM-1 in peripheral serum and the two gene polymorphism above. (7 ) There were no relationship between the two gene polymorphism above and other biochemical conclusions.Conclusion ( 1 ) The concentration of sICAM-1 in peripheral serum in GD group was high;( 2 ) The forth exon of ICAM 1 gene in Chinese Hans in northern China existed of polymorphism of 721G? A;A allele may relate to early onset of GD;(3 ) The sixth exon of ICAM 1 gene in Chinese Hans in northern China existed of polymorphism of 1405A? G;but it has no dependability to the age of onset of GD;(4 ) The two gene polymorphism above did not act by sICAM-1.