Studies Of Protective Effect Of Okam J201 On Bleomycin Induced Pulmonary Fibrosis In Rats

Objective to study the protective effect of Okam J201 on bleomycin induced pulmonary fibrosis in rats and its mechanisms.Methods 64 Wister rats were randomly divided into four groups: control, model, low dose (50mg/kg , treatment groupl) and high dose (100mg/kg , treatment group2) , with 16 rats in each group. Pulmonary fibrosis was induced by intratracheal instillation of bleomycin , while the control group was given normal saline in the same condition. From then on , the rats received daily Okam J201 50mg/kg BW in the low dose group, 100mg/kg BW in the high dose group by intragastric administration , while the other rats were given normal saline daily. On the 7th day and the 28th day 8 rats in each group were sacrificed and the lungs were isolated after intratracheal instillation. Histology changes of the lungs were evaluated by Hematoxylin and eosin (HE) stain and Masson stain , and the immunohistochemical techniques were used to investigate the changes of Fas , FasL MMP-9, TIMP-2.Results Alveolitis of two treatment groups was improved significantly as compared to that of the model group on the 7th day and 28th (P<0. 01) . On the 7th day the difference of pulmonary fibrosis is significantly between model group and control group (P<0. 01) , but there was no significant difference between two treatment groups and model group or control group (P>0. 05) .On the 28th day pulmonary fibrosis of two treatment groups was improved significantly as compared to that of the model group (P<0. 01). The expression of MMP-9, TIMP-2 was significantly lower in two treatment groups than those in model group on the 7th day (P<0. 01) . On the 28th day, the expression of MMP-9 has no significant difference among four groups (P>0. 05 ), but the expression of Fas, FasL, TIMP-2 were significantly lower in two treatment groups than those in model group (P<0. 01) . Both alveolitis and pulmonary fibrisis were all no difference between treatment groupl and treatment group2 (P>0. 05) , and the expression of Fas FasL, MMP-9 TIMP-2 was also no difference (P >0.05) .Conclusions Okam J201 can alleviate alveolitis and pulmonary fibrosis, and inhibite the expression of Fas, FasL, MMP-9, TIMP-2 of pulmonary fibrosis induced by bleomycin in rats, suggesting its possible anti-pulmonary fibrosis...