Clinical Significance Of Changes Of Serum TNF-α And IL-6 Levels In Senile Hypertensive Patients

IntroductionEssential hypertension (EH) is a syndrome with advancing blood pressure as principal clinical manifestation, the main pathologic change of EH is arterio-lar sclerosis, and evoking bleeding succeeding damage of critical organs such as heart, brain and kidney because of thrombopoiesis. The pathogenesy and a few pathologic process of EH still haven t illuminated completely up to now. In recent years, it is thinking highly that cytokines participate in the generation and development of essential hypertension.Cytokines are a species of infro - substance generated by leukocyte and other different cells in - vivo, which are protein or micromolecule polypeptide pros-sessing immunological regulation and effective function, and most of cytokines effect target cells evoking extensive biological effect by means of autocrine or paracrine. Two significant cytokines such as tumor necrosis factor - alpha(TNF -α) and interleukin (IL - 6 ) are multifunctional proinflammatory cytokine secreted by activated macrophage, and participating immunifaction and inflammatory reaction, which have a close relationship with increasing vascular resistance , damage of vascular endothelial cell and atheromatous plaque. The study investigates cytokines' variation and clinical significance in cardiovascular diseases such as essential hypertension through observation changes of serum TNF - α and IL -6 levels in senile hypertensive patients.Material and Method1. Source and group of patientsThe study population consisted of 67 hospital patients who had verificated hypertension, coronary artery disease or chronic cardiac failure after processing clinical diagnosis. 22 patients who had healthy medical examination were selected as controls.2. Collection and disposal of samplesUlnar vein blood 4 ml was collected from all subjects with empty stomach in the morning, which was packed into tubes. After 2 hour standing, the samples were centrifuged with 3500rpm/min for 6 minutes. The collecting supernatant fluid was conserved in refrigerator with — 70*^0 , and defrosted once determination.3. Determination of cytokines(TNF - a fP IL -6)Using Enzyme Linked - Immuno - Sorbent Assay ( ELISA ) measurement, the ELISA plate were coated with monoclonal antibody beforehand, and the detecting polyclonal antibody were marked with biotin. After adding sample, antibody marked with biotin and avidin marked with peroxydase, ELISA plate color with TMB coloration liquid. Figure of 0. D. have positive correlation with content of TNF - a or IL - 6 in sample. Then we can get corresponding concentration of TNF - a or IL - 6 on the basis of extinction value of samples after all extinction value of standard preparation and samples subtract null pore.4. Blood uric acid (UA) , blood fat (TG, CHO, HDL, and LDL) , fibrin-ogen (FIB) ,and blood pressure(SBP and DBP)All subjects had hemospasia from ulnar vein blood 2 ml with empty stomach in the morning. Determination of UA;After drawing off blood serum, the experimenter applied UA - TOCS colorimetry. Determination of blood fat: After in-funding EDTA - K2 into the anticoagulation tubes, we can take routine biochemistry determination. Determination of FIB;After anticoagulated with 3. 2% citrate sodium and 3. 28% natrium citrcum, the samples were detected by coagulation. BP measurement: Measuring right upper extremity arterial blood pressure by mercury column baumanmeter with sitting position, the tester takes SBP, DBP by Korotkoff.5. Statistical treatmentAll observation indexes were demonstrated by mean ± SD ( x ± s ). SPSSwindow 10.0 software was used to analyze variance, P less than 0.05 indicates statistic correlation, and P less than 0.01 indicates significantly statistic correlation.Results1. Baseline characteristicsThere was no significant difference in baseline characteristics between four groups on age, gender, smoke and body weight (P>0.05).2. Concentration measurement of blood pressure and haematobiochemistry in four groups patients' blood serum2.1 blood pressureSBP: B - group and C - group were obviously higher than A - group;DBP: B - group was higher than A - group, C - group and D - group.2.2 haematobiochemistryAll UA, FIB, TG, CHO, LDL increase guadually in A - group, B -group, C - group and D - group, corresponding with TNF — ou IL - 6, while HDL was opposite.3. CytokinesComparison with control group: The content of TNF - a ^ IL - 6 of three groups of trial are both higher than the control group (P<0.05 orP<0.01);Group comparison: TNF - ctAIL -6 ofD - group were obviously higher than that of B - group and C - group, and there was no statistic difference between B -group and C - group.4. The dependablity comparison between TNF -a^IL -6 and blood pressure , haematobiochemistry of all trial patients.TNF -a: Having positive correlation with SBP, DBP, TG, LDL, UA, and FIB( P <0. 05 or P < 0. 01) , and having negative correlation with HDL ( P < 0.05).IL -6: Having positive correlation with SBP, CHO, UA(P <0. 05 or P < 0. 01) , and having negative correlation with HDL (P <0. 05) .Conclusion1. The levels of TNF - a and IL -6 in senile hypertensive patients are higher than that of control group, which are higher following the addition of complications.2. There was correlation relationship between blood pressure, uric acid, blood fat, fibrinogen and cytokines.