A Study Of MMP In Ascites And Tissue Of Ovarian Carcinoma

Objective:Ovarian cancer is one of the leading mortality from gynecologicmalignancies. There is shifting and infiltration in early stage. The appearance of ascites implies abdominal shifting of ovarian cancer. First, the shifting and infiltration of the tumor make a breakthough basement membrane barrier, MMP-2 and MMP-9 can degrade type IV collagen which is the major component of the basement membrane, they are the importance in the shifting and infiltration of ovarian tumor. To determine whether elevated levels of MMP-2 and MMP-9 are present in ascites and in tissue from patients with ovarian cancer, and to analyze the relation between MMP-2 , MMP-9 and clinicpathologic features in order to guide clinical diagnosis and evaluate prognosis. Methods :(1) The level of MMP-2 and MMP-9 are detected from 50 ascites speciment of ovarian carcinoma, 16 of ovarian epithelial benign epithelial neoplasms, 7 of ovarian endometriotic cysts with ELISA.(2) The expression of MMP-2 and MMP-9 are detected 29 tissue speciment of ovarian carcinomal, 11 of ovarian benign tumor with immunohistoch-emiscal method.Results:(1) Patients in ovarian carcinoma group had significantly higher ascites MMP-2 and MMP-9 levels compared with the ovarian nonmalignant diseases group.(2) In ovarian carcinoma group, the level of ascites MMP-2 and MMP-9in ovarian malignant tumor are significantly higher in clinical UK IV stage than in clinical I -, II stage. The level of ascites MMP-2 is higher in poorly differentiated group or lymph node metastasis group or ascites amout exceeded 1000ml group or remain diameter exceed 2cm group than well differentiated group or no lymph node metastasis group or ascited amout lower 1000ml group or remain diameter lower 2cm group, but the difference is not significant. The level of ascites MMP-9 in lymph node metastasis group is higher than no lymph node metastasis group, but the difference is not significant. The level of ascited MMP-2 is not hign in poorly differentiated group or ascites amout exceeded 1000ml group or residue diameter exceeded 2cm group than well differentiated group or ascited amout lower 1000ml group or redidue diameter lower 2cm group, and the difference is not significant. There were no significant corr-elationship between the lever of ascites MMP-2 , MMP-9 and histological classifications.(3) The ascites levels of MMP-2 and MMP-9 were significant sensitive to ovarian carcinoma than ascites cytology examine. There was no significant sensitive between MMP-2, MMP-9 and serum CA125 examination.(4 ) MMP-2 and MMP-9 are expressed in both tumor and stromal cells, chiefly located in cell plasma. Their expression in cancer and in clinical IIL IV stage are enhanced compared to benign tumors and clinical I > II stage. Their expression in tumor cell was enhanced compareced to in stromal cells. Their expression of tumor tissue is concordant with ascites. Conclusions: Ascites MMP-2 and MMP-9 levels were significantly elevantedin ovarian malignant neoplasm, which might represent potential biomarker for ovarian cacinoma. The ascites level of MMP-2 and MMP-9 may evaluate prognosis in ovarian carcinom. The MMP-2 and MMP-9 chiefly originated from tumor cell.