| [Objectives] We investigated the relationship between T cell receptor Vβ subfamily clonal proliferation and GVHD through observeing the dynamic change of T-cell receptor Vβ subfamilies;And analysised the effect of Treg cell T cell subset and cytokine to GVHD through clinical datas. [Methods] 1. Amplification 24 TCR Vβ subfamilies with RT-PCR and secondary PCR were separated on agarose gel electrophoresis to analysis T cell clone.2. To detect T cell subset CD4+CD25+ cell and cytokine in different periods with flow cytometry and ELISA. [Result] 1 .Patients of HSCT were general pass through a transformational from monoclone to polyclone. The peripheral blood of GVHD patients mainly express monoclone/bioclone at the time of target organs damage conspicuously,after medication intervention,partial monoclone/bioclone expressed TCR Vβ subfamilies were diverted to polyclone expressed.2. There were significant difference of CD4+CD25+ cell , NK cell and cytokine level of IL-4, IL-10 sIL-2R between patients with GVHD and without GVHD after transplantion. [ Conclusion ] 1. T cell was clonal proliferated and T cell receptors were prone useed in early time post transplantion;And TVR Vβ subfamilies renewed to normal polyclonal along with haematogenesis and immunizing recovery .2. To detect CD4+CD25+ cell rate NK cell rate, and cytokine level IL-4, IL-10 sIL-2R can be usedto forecast the happening of GVHD.
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