Nuclear Factor KappaB,Telomerase Reverse Transcriptase,Cyclooxygenase-2 Expression And Clinic Significance In Human Laryngeal Squamous Cell Carcinoma

Objective: To investigate nuclear factor-kappaB/P65 (NF-κ B/P65) , telomerase reverse transcriptase (TERT), cyclooxygenase-2 (COX-2) gene expressions and clinical significance in human laryngeal squamous cell carcinoma. To investigate primarily the relationships between NF-κ B and TERT, NF-κ B and COX-2 gene expressions.Methods: Forty-one samples of laryngeal squamous cell carcinoma(LSCC) and ten samples of vocal cord polyps(VCP) were assayed using immunohistochemical SP method for detection of NF-κ B/P65, TERT, COX-2 protein expression level. To respectively compare the three protein expression differences between the LSCC group and VCP group, compare respectively the relationships between the protein expression and the major clinic indexs. To invest the relativities of NF- κ B and TERT, NF- κ B and COX-2. The datas are analysed by the SPSS11. 0 statistic soft package.Results: (1) There is significant difference(P<0.01) between the LSCC group and VCP group about NF- κ B/P65, TERT, COX-2 protein expression level, the positive expressions of NF- κ B/P65,TERT,COX-2 are respectively 68.29%, 78.05%, 73.17%. (2) Qualitative analysis finds that there' s significant relationship between COX-2 expression level and histological classification, also finds there' s significant relationship between TERT expression level and T grade, lymphnode metastasis, clinical stages in LSCC group. (3) There' s positive correlation expression level relationships between NF- κ B and COX-2, NF- κ B and TERT by using spearman correlation analysis.Conclusion: (1) NF-κ B/P65, TERT, COX-2 involve the development procession of laryngeal carcinoma. (2)TERT possibly becomes the target of preventing the LSCC deteriorism. (3) In LSCC, NF-κ B/P65 potentially promotes the expression of TERT and COX-2. (4) NF- κ B possibly becomes thenew target for treating LSCC.