Effects Of Testosterone On The Apoptosis Of Neurons And Astrocytes After Brain Ischemic Injury In Rats

Objective: to study the effects of androgen on the apoptosis of the neuron and astrocytes after cerebral ischemia injury. Methods: to investigation the apoptosis of astrocytes with TUNUL in adult male rats with orchidctomy and testosterone treatment. Result: 1) Observe of HE staining showed that there is no pathyology varity in normal group. But in the ischemic group neuronal necrosis can be found. Neuroral plasma concentrated, and its nucli disapeared. In the castrated+ischmia group, neuronal and astrocytes necrosis obviously be detected, neuronal and astrocytes nuclear crashed, forthmore, the apoptosis of above two cells beltly discovered this result accordance with TUNUL staining; 2)TUNUL staining showed that the apoptosis positive cannot be checked out in normal group but in all ischemia group, statistic revealed there are significant changes of apoptosis cell between group of ischemia 2 d and ischemia+castration 2d, ischemia 7d and ischemia+castration 7d, ischemia+ castration 2d and ischemia+castration+testosterone treatment 2 d (P<0.05). Conclusion: testosterone treatment can protect neurons and astrocytes after cerebral ischemia injury.