Effect Of Small Interfering RNA On The Expression Of Lung Cancer-associated Antigen Encoding Gene

Objective To study the effect of encoding gene of short hairpin RNA (shRNA) targeting alpha catalytic submit of mutant protein phosphatase 2A(PP2A) on GLC-82 cells with RNA interference technology. Methods Four shRNA targeting the mRNA of mutant protein phosphatase 2A were synthesized by in vitro transcription and transfected into GLC-82 cells with lipofection. After incubation for 48 hours, we observed and evaluated the cells morphological change , the survival rate of the cells with MTT assay and the protein expression with Western blot. Results Compared with the blank control, the four shRNA have not the same effects, One shRNA suppressed cell proliferation and appeared the cells apoptotic morphological change, and exhibited obvious effects on inhibiting the protein expression of GLC-82 cells. Conclusion One shRNA of the four were obtained, which could specially and effectively inhibit the expression of alpha catalytic submit of mutant protein phosphatase 2A inGLC-82 cells. It may open a new approach to using shRNA as a new tool to further study this gene' s function and mechanisms, and may be developed to be a new gene therapeutic agent for Lung cancer.