The Expression Of Endogenous β-gal And GFP And The Interference Function Of Them Under Lipid Levels In Rats

Objective To supply reference for selecting reporter gene which was used in gene target system, the variety of different lipid levels of the endogenous expression β -gal, GFP were compared in rats. Methods 16 male SD rats were divided into two groups , control and higher lipid group which were feed with higher lipid feed stuff until their lipid increasing .The rats were sacrificed and 11 organs were detected for β -gal activation and GFP by enzyme histochemistry, fluorescence, respectively. Results The positive expression of β -gal were in liver, spleen, lung, kidney, brain, lymphoid, thyroid, intestine; The negative expression of β -gal were in vascular-breast aorta , heart, diaphragm. The spontaneous green fluorescence were detected in breast aorta , liver, spleen, lung, kidney, thyroid, diaphragm and no spontaneous fluorescence in heart, brain, lymphoid, intestine. All results were consistent under different lipid levels. Conclusion 1 Under classical X-gal staining, β -gal can be used asgene reporter in vascular, heart, diaphragm in which endogenous β -gal is negative expression. But β -gal can't be used as gene reporter in liver, spleen, lung, kidney, brain, lymphoid, thyroid, intestine because of it's positive expression of endogenous β -gal. High lipid levels has no effect on the endogenous expression of β -gal in rats. 2 There are no spontaneous green fluorescence in heart, brain, lymphoid, intestine which can be used as gene reporter , in contrast, there are spontaneous green fluorescence in breast aorta , liver, spleen, lung, kidney, thyroid, diaphragm which can't be used as gene reporter . High lipid levels has no effect on the endogenous expression of GFP in rats.