The Study Of Roles About Syk Promoter Methylation In Postoperative Recurrence And Metastasis Of Hepacellular Carcinoma

Objective To detect methylation status of the promoter region ofSyk gene in hepatocellular carcinoma(HCC),the relationship betweenthe aberrant methylation of Syk gene and postoperative recurrenceand metastasis after resection of HCC was analyzed.Methods The samples of tumor and adjacent normal tissues wereselected from HCC patients(n=64).They all underwent resection,44cases underwent radical resection and 20 cases underwent palliativeresection. Total genomic DNA was extracted from tissue specimens,then DNA was treated with bilsulfite modification. Finally, usingmethylation-specific polymerase chain reaction technique (MSP),specimens from 64 HCC patients (tumor tissues, adjacent normaltissues) were detected methylation of Syk promoter region. Duringfollow-up, 33 cases were found to have postoperative recurrence andmetastasis. According to these data, we analyzed the relationshipbetween the aberrant methylation of Syk gene and postoperativerecurrence and metastasis.Results ⑴Among 64 HCC, methylation of Syk promoter region werefound in 19 patients(29.7%). In contrast, methylation of Sykpromoter region were found in only 2 of adjacent normal tissues(3.1%), the rate of methylation of Syk promoter in HCC was higherthan that of adjacent normal tissues. ⑵In 19 cases with Sykpromoter methylation, 14 were found to have postoperativerecurrence and metastasis(73.7%).In 45 cases without Syk promotermethylation, only 19 were found to have postoperative recurrenceand metastasis(42.2%). A significant difference was noted betweentwo groups .Conclusion ⑴There is significant difference of Syk promotermethylation between HCC and adjacent normal tissues. ⑵Promotermethylation of Syk gene may serve as a potential biomarker forpostoperative recurrence and metastasis of HCC patients.