| RT-nested PCR and DNA sequencing were used to genotype of samples of HFRS of rattus norvegicus and patients sera from fujian province. Selected parts of the samples' PCR products were purified for sequence analysis. The results showed that: 23 samples from 24 HFRS rattus norvegicus were tested positively by M gene primers and RT-nested PCR. The positive rates were 95.8%; Only 11 samples can amplified from 20 serum samples of HFRS patients, which positive rates were 83 % (≤ 1W), 12.5% (>1W) respectively. There was only one Hantaan virus between the positive samples of amplification by RT-nested PCR, which was the same as the results of sequence analysis. It shows that Seoul virus is a predominant serotype in fujian province. Compared with the sequence, we found that in the same district,the identity of the nucleotides between Seoul viruses is very high(>99%). But there was large diversity between different districts, especially in yuanchun and songxi, which was less than 20% of identity of the nucleotides. They are possible new subtype of Seoul virus.The NP recombinant antigens of A537 were expressed in E.coli. The expressesd recombinant proteins were purified by electroelution or Ni-NTA affinity chromatography. The purified proteins were applied in an indirect ELISA by coating the polystyrene 96 well plate to detect IgG in HFRS infection. Compared with IFAT, the indirect ELISA showed 94. 7% in sensitivity and 95. 6% in specificity, which is suitable for epidemiological investigation. The immunochromatographic assay applying the purified recombinant antigen conjugated with colloidal gold as the capture reagent can detect IgM and IgG from HFRS infection in the same time, with 97.9% in sensitivity and 98.2% in specificity in comparing with IFAT/ELISA. The immunochromatographic assay can be applied in quick diagnosis for clinical patients in all conditions , especially in the countryside or hospitals without basic experimental equipments.
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