A Study On The Repair Of Canine Experimental Class Ⅱ Furcation Defects By Tissue Engineering

Objective:　1.The　purpose　of　the　first　study　was　to　assess　　bone　marrow　stromal　cells(BMSCs)and/or　platelet-rich　plasma(PRP)　auto-transplanted　to　promote　periodontal　regeneration　in　canine　experimental　classⅡfurcation　defects.　2.To　compare　the　difference　in　promoting　periodontal　regeneration　of　initial　cell　seeding　density　in　canine　experimental　class　Ⅱfurcation　defects　.　Methods:　1.A　total　of　30　experimental　classⅡfurcation　defects　were　randomly　divided　into　four　groups　and　were　surgically　repaired　by　GTR,GTR/PRP/Bio-Oss,GTR/BMSCs　and　GTR/BMSCs/PRP/Bio-Oss.The　animals　were　sacrificed　at　12　weeks　postsurgery　for　histologic　and　histometric　analysis.　2.GTR　combination　with　the　expanded　BMSCs　which　were　seeded　in　collagen　membrance　at　initial　concentrations　of　5×105cells/ml,5×106　cells　/ml　or　5×107　cells　/ml　,and　auto-transplanted　into　experimental　class　Ⅱfurcation　defects,underneath　a　Bio-Gide　membrane.　Bio-Gide　membrane　alone　was　implanted　into　the　defects　as　a　control.Periodontal　regeneration　was　evaluated　by　histologic　and　histometric　analyses　12　weeks　after　transplantation.　Results:　1.Significantly　greater　periodontal　regeneration　was　noted　in　the　groups　of　PRP/BioOss/　BMSCs　/GTR　compared　with　the　PRP/BioOss/GTR,　BMSCs　/GTR,　GTR　treatment　alone　when　newly　formed　periodontal　tissues　including　alveolar　bone,　periodontal　ligament　and　cementum　were　assessed.　Interestingly,　no　significant　difference　was　found　between　GTR　alone　or　plus　BMSCs　　or　GTR　combine　with　of　PRP/Bio-Oss.　The　amount　of　new　alveolar　bone　formation,　new　cementum　formation,　new　periodontal　ligament　tissue　formation　in　the　PRP/BioOss/　BMSCs　/GTR　group　was　1.75±0.840mm,2.271±0.704mm,2.606±0.410mm,　　respectively.　The　GTR　group:　0.737±0.487mm,1.149±0.675mm,1.514±0.905mm;　the　PRP/Bio-Oss/GTR　group:　0.494±0.564mm,1.414±0.638mm,1.557±0.772mm;　the　GTR/　BMSCs　group:　0.82±0.576mm,1.657±0.619mm,1.871±0.670mm.　2.　The　percentage　of　new　cementum　length　in　the　5×105cells/ml,5×106　cells　/ml　and　5×107　cells　/ml　groups　were　significantly　higher　than　in　the　control　group　(P<0.01),　and　there　was　no　significantly　difference　in　the　three　cell　groups.　The　percentage　of　new　alveolar　area　in　the　5×106　cells　/ml　and　5×107　cells　/ml　groups　were　significantly　higher　than　in　the　control　and　5×105cells/ml　group　(P<0.01),　and　there　was　no　significantly　difference　between　the　first　two　groups,and　neither　was　the　later.　　Conclusions:　1.The　data　from　the　first　study　suggest　that　the　application　of　GTR　combination　with　PRP　and　BMSCs,but　not　alone　can　support　the　periodontal　tissue　regernation　in　canine.　2.The　second　study　suggest　that　the　effect　of　auto-transplanted　BMSCs　promoting　periodontal　healing　has　cell　density–dependent　under　certain　conditions,and　the　higer　concentrations(5×106　cells　/ml　and　5×107　cells　/ml)　can　　effectivly　promote　periodontal　regeneration.