Effect Of Mifepristone On IGF-1, IGF-1R, Mast Cells And Ultrastructure Of Human Fallopian Tube

Objective:1. To observe the effect of mifepristone on the expression of insulin-like growthfactor-1( IGF-1) and its receptor (IGF-1R) during the med- or late proliferative phase of menstrual cycle in the epithelium of human fallopian tube.2. To investigate the effect of mifepristone on mast cell (MC) number of human fallopian tube.3. To investigate the effect of mifepristone on the ultrastructure of the epithelium of human fallopian tube.Methods:1. Fallopian tube in med- or late proliferative phase of menstrual cycle was taken from patients of reproductive age. Patients of mifepristone group (n=8) took a single dose of mifepristone 25mg, 12h preoperatively. No mifepristone was given to patients of control group (n=7). Expression of IGF-1 and its receptor was identified using immunhistochemistry and image analysis method.2. Fallopian tube was taken from patients of reproductive age. Patients of mifepristone group (n=16) took a single dose of mifepristone 25mg, 12h preoperatively. No mifepristone was given to patients of control group (n=13). The distribution and number of MC were examined by means ofimmunohistochemistry and image analysis system.3. Fallopian tube was taken from patients of reproductive age. Patients of mifepristone group (n=6) took a single dose of mifepristone 25mg, 12h preoperatively. No mifepristone was given to patients of control group (n=5). The ultrastructure of epithelial cells of fallopian tube were observed withTEM. Results:1. During med- or late proliferative phase of the menstrual cycle, the expressionof IGF-1 and IGF-1R showed no significant difference in the epithelium of isthmus, ampulla and fimbrium in both groups (P>0.05); and no significant difference of the expression of IGF-1 was found in the epithelium of the same portion of fallopian tube between mifepristone group and control group (P>0.05); while under the effect of mifeprostone, the expression of IGF-1R in the epithelium of the same portion of fallopian tube was significant increased as compared with control group (P<0.05).2. In the control group, the MC number in the same portion of muscular layer and mucosa showed no significant difference during the menstrual cycle (P>0.05). The MC number in muscular layer was decreased in sequence as isthmus> ampulla> fimbrium (P<0.05), but no significant difference of MC number was found between the ampullar and fimbrial mucosa. (P>0.05). In the mifepristone group, during the menstrual cycle, the MC number in the muscular layer of isthmus and ampulla, but not fimbrium, was significant decreased (P<0.05), as compared with control group. In the mifepristone group, during the menstrual cycle, the MC number in the ampullar, but not in the fimbrial mucosa, was obviously less than that of control group (P<0.05).3. In comparison with the control groups, the epithelium of fallopian tube of mifepristone group displayed the following morphological change:â‘  cilia were edematous, the roots on the base body of cilia were rare or shorten; microvilli were reduced;â‘¡ mitochondria were vacuolated;â‘¢ autophagic vacuoles, lipofuscins and lipid droplets were often seen in cytoplasm.Conclusion:1. During the med- or late proliferative phase of the menstrual cycle, there was no significant difference of expression of IGF-1 on the epithelium of human fallopian tube between mifepristone group and control group, while the expression of IGF-1R was obviously increased under the effect of mifepristone. These indicate that mifepristone might affect the environment of human fallopian tube.2. The MC number of the tubal muscular layer and mucosa is not dependent on the menstrual cycle. Under the effect of mifepristone, the MC number in the wall of the Fallopian tube, except fimbrium, is less than that of control group. Since MC can reduce blood flow and inhibit the contractible function of smooth muscle cells, mifepristone might affect the transfer of fertilized ovum.3. Single low does of mifepristone can impact on the ultrastructure of the epithelium of human fallopian. Therefore, it might affect the fertilization and development of embryo via changing the environment of pre-implantation, also, it might affect the transfer of fertilized ovum.