Expression Of Survivin,CGRP And Achr In Rats With Cerebral Ischemia/Reperfusion

[Objective] To observe the vary regulation of surviving CGRP and AchR, To explore the effects of survivin, CGRP and AchR on brain after damaged .[Metheds] In the present study, immunohistochemistry, HE stain were used in a focal cerebral ischemia- Reperfusion model with middle cerebral artery occlusion in 36 healthy adult Wistar rats to examine the expression profile of surviving CGRP andAchR.[Results] In low magnification of HE stain, neuron in central area necrosis and in semi-sepheral area decreased and degeneration: cell body was triangle, sector, long-strip, with karyopyknosis, deep staining of nucleolus, and neoplasm was vacuoles. Cortex neuron was neoplasm-rarefaction, nuclea-malformation slightly and deep-staining. Degenerated neurons and normal neurons were coexist. There were lots of neurogangalions in both the cetral area and semi-sepheral area. Our results also indicated that the expression of survivin in the cerebral cortex was increased after cerebral ischemiae compared with pseudo-operation. The results showed that the first peak was at 6 hours after reperfusion, decreased at 24h, and then the second peak at 3d, and the second peak was lower than the first one, declined gradually, reached the level of background in 14d. While its expression in striatum increased from 2h to 7d, and the only peak was at 3d, finally reached to background level at 14d. The expression of CGRP in the cerebral cortex was increased after cerebral ischemia compared with pseudo-operation, there was three peaks respectively at 2h and 6h , 48h, 7d, and then decreased gradually to the background level afterwards. While its expression in striatum reached to two peaks at 2h and 3d, 7d. Then decreased gradually to the level of ischemia in initial stage. The expression of AchR in the cerebral cortex was also increased after cerebral ischemia compared withpseudo-operation, there was also two peaks at 6h , 3d and 7d. While in striatum was two peaks at 2h and 3-7d.[Conclusion] The expression of survivin, CGRP and AchR all increased apparently in brain injury, they were related to the re-prosthesis of nevous tissue after damaged.