Genotyping Of Isolated Chlamydia Trachomatis Strains And DNA Sequence Polymorphism Of Chlamydia Trachomatis Omp1 Gene

Objective:To investigate the distribution of Chlamydia trachomatis serovars and characterize the Ompl gene by sequencing DNA from detected cases of Chlamydia trachomatis infection in partial cities in China, in order to understand the epidemiological characterization of Ct infection. Methods:The major outer membrane protein gene (Ompl) of Chlamydia trachomatis was amplified by nested polymerase chain reaction(nPCR) from clinical specimens collected in Hengyang, Shanghai, Guangzhou and Jiangmen, and restriction fragmemt length polymorphism(RFLP) pattern were analysed after Alu I and Msp I, EcoR I, Dde I digestion. The reference sequences were obtained from GeneBank and the enzyme cut sites were analysed by DNAstar software.Partial clinical isolates were sequenced, and multiple alignment was performed with CLUSTAL X. Then a phylogenetic tree was constructed by Mega 3 to illustrate the evolutionary relationships between clinical isolates and reference strains.Results:301 specimens were positive by cell culture, and 286 specimens (48 collected from Hengyang, 32 from shanghai, 52 from Guangzhou and 154 from Jiangmen) were amplified and genotyped by RFLP. The most prevalent genotype was serotype E (30.8%) , followed by J (23.8%), D (17.5%), F (10.5%), G (6.2%), H (3.5%), K (2.8%), Ba (2.1%), Da and I (1.4%). No difference was observed among different cities.