Measurement Of NO In Synovia Of Temporomandibular Disorders And Clinic Research

Objective: To study NO activity in synovia of temporomandibular disorders (TMD) patients with fluorometric assay, to observe the change of NO content in synovia before and after lavaging joint upper cavity, to understand the function of NO in pathological change of TMD, and to reveal the relationship between NO and TMD.Method: Chose 77 TMD patients in temporomandibular disease clinic of Second Hospital of Tianjin Medical University, who were divided into 3 groups: synovitis group, disc anterior displacement group and osteoarthropathy group, according to clinical examination, panoramic transcranial views of closed mouth and contrast examination. 10 normal volunteers as a control group. Collected temporomandibular synovia before and after treatment, centrifugated them for 10 minutes at 3000 r/min, and preserved the upper clear liquid at -70°C to be measured. Dyed the deposit with HE then made smears, which were observed under the optical microscope. Measured the change of NO content in synovia before and after lavage with fluorescence spectrometer.Results: 1. Only 1 case was measured NO in synovia in 10 volunteers, whose content was 0.17μmol/L.2. Before treatment there existed remarkable difference in NO content in synovia between disc anterior displacement group and synovitis group, disc anterior displacement and osteoarthropathy group (P<0.05) , but not between synovitis group and osteoarthropathy group (P>0.05) . Remarkable difference in VAS existed between articular forward shift group and synovitis group, but not between synovitis group and osteoarthropathy group. For the three groups therewere no obvious difference in mouth opening > protrusion+left+right +lateral jaw movement o3. For each of the three groups, there existed remarkable difference in NO content, VAS, mouth opening^ protrusion+left+right +lateral jaw movement before and after treatment.4. Observed under microscope, crystal and protein condensation could be found in synovia deposit before treatment, sporadically with neutrophil cells and lymphocyte, but after treatment only red undefined matter could be found, scarcely with cellsConclusions: 1. NO content in synovia of TMD patients is higher than that of normal people, and NO contents of synovitis group and osteoarthropathy group are higher than that of disc anterior displacement group, which indicated that NO participates in inflammatory action process and plays an important role in pathological process of TMD.2. Remarkable difference in VAS existed between disc anterior displacement group and synovitis group, but not between synovitis group and osteoarthropathy group. Experiment results indicate that VAS increases markedly in the group with high NO content. Correlation analysis, though, shows that VAS is not correlated to NO ( Y=0.008,P=0.946) .3. Compared with those before treatment, NO,VAS, mouth opening protrusion+left+right +lateral jaw movement in three groups varied markedly after lavage (PO.01) .after treatment, NO content and VAS decrease, but mouth opening protrusion+left+right +lateral jaw movement increase. Correlation analysis shows NO is irrelevant to VAS, mouth opening protrusion+left+right +lateral jaw movement and degree of pain...