The Effect Of Abnormal Chromosome In The Development Of The Mammary Phyllodes Tumours

Comparative genomic hybridization (CGH) is a powerful new molecular cytogenetic technique that can character excess and missing cytogenetic material in a one step global screening procedure. CGH provides an overview of DNA sequence copy number changes (losses, deletions, gains, amplifications) in a little tumor specimen and maps these changes on normal chromosomes for the different phase of malignant process, different developing tumour and different type rumours.The mammary phyllodes tumours (PTs) are classified three types: benign, bordline and malignant PTs, which reflect the change in the malignant process of phyllodes tumours and so they are applicable to CGH analysis.Objective: To analyze the DNA sequence copy number change in mammary phyllodes tumours from the benign to the malignant and provide a melocular cytogenetic marker with clinical and pathological diagnosis.Methods: CGH was used to analyse chromosomal copy number changes in 3 cases of benign PTs, bordline PTs and malignant PTs , respectively.Result:1. Average chromosome copy number changes detected in benign, bordline and malignant PTs were 8, 19, 25 respectively.2. DNA sequence copy number changes in benign PTs: Genomic losses prevalently involved chromosome arms 9q34 (2/3) .3. DNA sequence copy number changes in bordline PTs: Genomic gains prevalently involved chromosome regions 4p, 9q, 13q, 14q (2/3); Genomic losses prevalently involved chromosome regions 8q, 9q, 12q, 15q, 16q, 20q, 21q, 22q (2/3); High-level amplifications were detected on chromosomal...