| Objective: Study the mechanism of thrombogenesis and vascular occlusion which result from endovenous irradiation with the laser by means of the endovenous irradiation in the cephalic vein of the hybrid dogs with semi-conductor laser treatment instrument. Methods:30 dogs with identical weights were chosen and were divided into three groups of A, B, C at random.the forelegs of the animals of group A were divided into two groups, left forelegs: cut open the skin at 2cm of interior anodic of inner malleolus by the way of lateropulsion, and then reveal and free the cephalic vein, and then prick into the distal cardia extrem with No.19 trochar, and then draw the transfixion pin and detain the trochar, and then insert the 15cm-long optical fiber under the guide of nfrared light and emit the laser with the laser meter, exit the optical fiber at the speed of 0.5cm/s at the same time, and after 15 minutes cut open the skin of dogs and remove the vessel which was irradiated by laser and fix it in the 10% formalin. Right forelegs: Firstly make the vessels in which the normal sodium is. cut open the skin at 2cm of interior anodic of inner malleolus by the way of lateropulsion, and then reveal and free the cephalic vein, ligate the distal extrem, and then cut open the skin at 15cm of proximal extrem by the way of lateropulsion, reveal and free the cephalic vein, expose 1cm of it, ligate the proximal extrem and pink a hole at the proximal extrem with NO.12 bodkin, and then inpour the normal sadium at the distal extrem and douche the vessel, ligate the proximal, inpour the normal sodium at the distal extrem in order to make it reach the thickness of original vessel, and then prick into the distal cardia extrem with No.19 trochar, and then draw the transfixion pin and detain the trochar, and then insert the 15cm-long optical fiber under the guide of nfrared light and emit the laser with the laser meter, exit the optical fiber at the speed of 0.5cm/s at the same time, and after 15 minutes cut open the skin of dogs and remove the vessel which was irradiated by laser and fix it in the 10% formalin. The forelegs of the dogs in group B were divided into two groups, left forelegs: cut open the skin at 2cm of interior anodic of inner malleolus by the way of lateropulsion,and then reveal and free the cephalic vein, and then prick into the distal cardia extrem with No.19 trochar, and then draw the transfixion pin and detain the trochar, and then insert the 15cm-long optical fiber under the guide of nfrared light and emit the laser with the laser meter, exit the optical fiber at the speed of 0.5cm/s at the same time, suture the incision and bandage with pressure bandages. Right forelegs: press the vessel wall under the irradiation of laser, the other operations were the same as those of group of left forelegs. The operations of group C were the same as those of group B.Recheck the Plt, blood calcium, profibrin of the animals in group B and C on the second day after the operation. Remove the blood vessels of animals in group B which is irradiated by the laser on the fourth day after the operation and fix it in the 10% formalin. Remove theblood vessels of animals in group C which is irradiated by the laser on the eighth day after the operation and fix it in the 10% formalin. Observation of vessel wall morphology: get the sample in the 10% formalin, dehydrate it with alcoholic, embed it with paraffin and cut sheet, and observe the changes of histomorphology under the light microscope after HE stain. Observation of intravascular thrombogenesis.: get the sample in the 10% formalin, dehydrate it with alcoholic, embed it with paraffin and cut sheet, and observe intravascular thrombogenesis under the light microscope after HE stain. Results: Normal control: The cross section of vein presents the oval, the inner membrane is composed of a layer of endothelial cells. The inner membrane is smooth and integrity, the vein wall has no obvious thickening. The outer membrane is constitute by tela conjunctiva, there were few longitudinal smooth muscles in it. edema and hemorrhage were not seen in the vessel wall, there was no inflammatory cell infiltration around blood vessel and there was little blood blot in the vessel cavity. Group A: The endangium of cephalic vein of left forelegs hurt obviously, the bulk of endothelial cells fell off and little mural thrombus was formed in the vessel. The vein wall presented obvious dropsy, the bigger leakage appeared among the smooth muscles, dropsy appeared around the blood vessels and there was no inflammatory cell infiltration; The endangium of cephalic vein of right forelegs is a layer of endothelial cells, endomembrane issmooth, only little defect could be seen. obvious thickening ,edema and hemorrhage were not seen in the vessel wall, there was no inflammatory cell infiltration around blood vessel and there was no blood cells in the vessel cavity. Group B: The endangium of cephalic vein of left forelegs hurt, the big part of endothelial cells fell off, the bulk mural thrombus was formed in the vessel and the vessel cavity was almost filled with it. The vein wall presented dropsy, the bigger leakage appeared among the smooth muscles, partial endangium was carbonized without perforation of the vein wall, dropsy appeared around the blood vessels. there was a small mounts of inflammatory cell infiltration in the vessel wall and thromb, they are mainly granulocyte and lymphocyte; The endangium of cephalic vein of right forelegs hurt, endothelial cells fell off and disappeared, the bulk mural thrombus was formed in the vessel and the vessel cavity was almost filled with it. The vein wall presented dropsy, the bigger leakage appeared among the smooth muscles, partial endangium was carbonized with perforation of the vein wall, dropsy appeared around the blood vessels. there was a small mounts of inflammatory cell infiltration in the vessel wall and thromb, they are mainly granulocyte and lymphocyte; Group C: The endangium of cephalic vein of left forelegs hurt, the big part of endothelial cells fell off, the bulk mural thrombus was formed in the vessel and the vessel cavity was almost filled with it. The vein wall presented dropsy, the bigger leakage appeared among the smooth muscles, partial endangium was... |
