| Objective: To investigate the mechanism of acupuncture and moxibustion in anti-aging effect. Methods: 32 mice were randomly and evenly divided into control, model, model+acupuncture and model+moxibustion groups. Subacute aging model was established by subcutaneous injection of D-galactose .We detected the change of superoxide dismutase(SOD) and malondialdehyde (MDA) of serum and thymus tissue. We also detected the level of nitric oxide (NO) and expression of bcl-2 in thymus tissue. Results: In the thymus tissue and serum of the aging mice(model group), MDA content increased remarkably while SOD activity and expressions of bcl-2 lowered apparently, compared with those of control group (P<0.05). After acupuncture treatment, the increased content of MDA lowered significantly while the decreased SOD activity and expression of bcl-2 raised considerably in comparison with those of model group(P<0.01 or P<0.05), but had no marked difference with control group (P>0.01 or P>0.05). About model+moxibustion group, the change of MDA content, SOD activity in serum and expressions of bcl-2 in thymus were close to model+acupuncture group, but SOD activity in thymus tissue had no marked difference with those of model group. Conclusion: Acupuncture and moxibustion have effect in resisting thymus aging by way of inhibiting reaction of free radical and raising the activity of antioxidase and up-regulating the expression of bcl-2.
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