Establishmert Of Camptothecin Resistant Human Stomach Cancer Cell Line SGC-7901/HCPT And Analysis Of Biological Properties

Objective:To establish the camptothecin (HCPT)-resistant stomach cancer cell line SGC-7901/HCPT with its characteristics studied. Method: Cell growth curve was painted and the doubling time was accouted by cell counting assay; the Camptothecin-resistant stomach cancer cell line SGC-7901/HCPT was established by repeatedly exposing stomach cancer cell line SGC-7901 to increased gradually concentration of HCPT . The resistant index of the resistant cell line to different chemitherapeutic drugs was determined by the methyl thiazolyl tetrazolium assay (MTT) . Cell cycle distribution was measured by flow cytometry(FCM). Result : The HCPT-resistant human stomach cancer cell line SGC-7901/HCPT was established after 9 months. the rate of cell proliferation of SGC-7901/HCPT was longer than that of SGC-7901 SGC-7901/HCPT indicated drug-resistance to HCPT,the drug resistant index being 8.919, but are not cross-resistant to Mitomycin C(MMC), Cisplatin (CDDP), 5-fluorouracil5 (5-Fu), Vincristine sulfate(VCR), Adriamycin (ADM), Daunorubicin(DVR), Etoposide(Vp-16). the mumher of cells in S phace and G2-M phace increased( P<0.005), suggesting that the DNA cynthesis was increased in SGC-7901/HCPT. Conclusion: the Camptothecin-resistant stomach cancer cell line SGC-7901/HCPT was established, It can be used for further experiments.