Evaluation Of Albumin Cobalt Binding Assay And Its Preliminary Assessment Of Acute Coronary Syndrome

Objective To investigate the experiment conditions of albumin cobalt binding assay (ACB assay). And to evaluate the capacity of human albumin to bind cobalt in patients with myocardial ischemia. Methods This study established the experiment conditions of albumin cobalt binding assay (ACB assay), included the wave length of max absorbance, eluting peak and retention time, standard curve, linearity of the ACB assay. Plasma samples were tested for the ACB assay on 76 subjects with suspicious acute coronary syndrome. The 76 subjects were divided into acute coronary syndrome(ACS) group and non-ACS group according to the results, ACS group included UA and AMI. And comparison ACS group with non-ACS group, UA group with non-ACS group, AMI group with non-ACS group ,UA group with AMI group, respectively. Results The established conditions of ACB assay in our study. The max absorbance was 480nm.The time of eluting peak was 10 minutes, and persisted 15 minutes, standard curve equation was Y=-0.035+1.548X, linearity of the ACB assay was over a concentration range of 20-60 g/L, the regression equation was Y=1.229-0.006X, the correlation coefficient was 0.993. ACB assay result in 76 patients. The absorbance unit(ABSU) for ACS group and UA group were significantly greater than it for non-ACS group(P﹤0.01). While there were no siginificantly difference between MI group and UA group(P=0.175). And even adjusted the concentration of albumin, the results were similar. Conclusion The ACB assay is simple,rapid,accurate and suitable for routine use in clinical laboratories. The capacity of human albumin to bind cobalt is reduced in myocardial ischemia .IMA is a clinical useful marker to identification suspicious ACS .