Establishment Of Analysis The Methylation Of FMR1 And XIST Genes Detection

Objective: To establish time-efficient and sensitive method for detection the methylation of FMR1 and XIST genes.Methods: Genomic DNA was deaminated by sodium bisulfite,two sets of PCR primers which are specific to unmethylated and methylated DNA respectively ,were used to amplify the target regions of FMR1 and XIST. The PCR products were further cloned and sequenced.Results: The sequence of PCR products were identified by cloning and sequencing.Unmethylated cytosine residues (C) converse to uracil (U), while methylcytosine (mC) was not modified under the condition used.Conclusion: In the present study,a new method of detectionof the methylation of FMR1 and XIST genes was successfully established, it may apply a new technique for clinic dignosis for Fragile X syndrome.