Comparative Analysis Of Fluoresenes Quantitative-PCR And Reverse Transcription-PCR In Detecting HCV-RNA

AIM: To investigate the clinical significance of the fluoresenes quantitative - PCR (FQ-PCR) and reverse trascribtion PCR(RT-PCR) in detction of serum HCV-RNA. METHODS Total 1062 patients with anti-HCV positive were detected HCV-RNA, in 481 patients were using RT-PCR, and in 465 patients were using FQ-PCR .We also detected in 116 cases using two PCR methods simultaneously. RESULTS The positive rate of RT-PCR was 49.90%, and FQ-PCR was 62.58%. The detected range of HCV RNA was from 10~3 to 10~8 copies/ml.The rate of simultaneously positive was 49.14% by RT-PCR and 65.22% by FQ-PCR, respectively. The positive rate of FQ-PCR was significantly higher than by RT-PCR . In 40 cases of negative by FQ-PCR were foud 4 positive cases by RT-PCR. CONCLUSION Compared with two methods, the positive rate of FQ-PCR was higher than RT-PCR in detecting HCV-RNA, but the sensitivity of RT-PCR higher than the FQ-PCR.A few negative cases by FQ-PCR cant exclud HCV-RNA positive probability.