Study On The Diagnostic Value Of Bile K-ras Motation In Animal Model With Hepatic Metastasis In Pancreatic Cancer

Objective: To investigate the value of K-ras mutation in bile for the earlydiagnosis of hepatic metastasis in pancreatic cancer.Methods: Buliding pancreatic cancer and hepatic metastasis in pancreaticcancer of animal model with rats ,we collected bile from them ,and used thepolymerase chain reaction and restriction fragment-length polymorphism(PCR-RFLP) to evaluate K-ras point mutation at code 12 in bile. Then, theresult was compared with pathologyic slice up..Results: The positivity of K-ras point mutation in bile of hepatic metastasis inpancreatic cancer was 84.2%(16/19), and that was 0.0%(0/16) in model withpancreatic cancer.The sensitivity of surveying K-ras mutation in bile of hepatic metastasis inpancreatic cancer was 66.7%, the specificity was 81.0%, the positive predictionwas 80.0%, and the negative prediction was 85.0%.Conclusions: It suggests that K-ras point mutation at codon 12 in bile is athigh risk for development of hepatic metastasis in pancreatic cancer. Both themutability and the specificity of K-ras are high .Our work can supply a newelemental filter method for the early diagnosis of hepatic metastasis in pancreatic cancer.