The Effect Of Angiotensin Ⅱ And AT1,AT2 On Pathological Scar

Purpose: By testing the expression of angiotensin Ⅱ and its receptor in pathological scar and pathological skin, We will discuss the relationship between angiotensin Ⅱ and the formation of pathological scar. Material and method: The sample of burns and plastic surgery patients and the liver sample of hepatocirrhosis are provided by pathology of West China Center of Medical Science of Sichuan University. Normal skin sample :11 set (Taken from the normal skin in plastic surgery). Hypertrophic scar :11 cases, Keloid sample 11 cases, hepatocirrhosis :2 cases, 35 in total。 We use the Immunoenzymcytochemistry to test the expression and location of AngⅡ and AT1 , AT2 in normal skin and pathological scar. We employee the blindmethod to test positive reaction and classify them into 4 classes by thestaining condition in order to further discussion. We also use the SPSSstatcistical software set to analyze the result.Result: Positive control shows the strong positive, and blank control showsnegative.(1) The expression of angiotensin Ⅱ is negative or doubtfully positive in normal skin, and positive or strong positive in hypertrophic scar and keloid. There is different expression of Ang Ⅱ between normal skin and pathological scar statistically(P<0.05) but no different between hypertrophic scar and keloid statistically(P>0.05) .(2) The expression of AT1 is negative or doubtfully positive in normal skin, and positive or strong positive in hypertrophic scar and in keloid. There is significant difference(P<0.05) between the expression of AT1 in normal skin and pathological scar but no statistical difference between its expression in hypertrophic scar and keloid. (P>0.05)(3) The expression of AT2 is negative or doubtfully positive in normal skin, and positive or strong positive in hypertrophic scar and in keloid. There is different expression of AT2 between normal skin and pathological scar statistically(P<0.05) but no different between hypertrophic scar and keloid statistically(P>0.05) .Conclusion: (1) Angiotensin Ⅱ may have effect in maintaining the physiological function of normal skin.(2) Angiotensin Ⅱ is involved in the formation of scar.(3) ATI together with AT2 mediate the function of angiotensin II in scar formation.