Studies On Multiplex PCR For STR Loci & Correlation Between STR Loci And Human Esophageal Cancer

Objective: To establish a multiplexing method of three STR loci (D3S1754,D4S1647 and D12S391). Method: A multiplexing heat-soaked PCR amplification system of three short tandem repeats loci (D3S1754, D4S1647 and D12S391) , denaturing polyacrylamide gel electrophoresis (DPAGE) and silver staining were used. Result: The multiplexing method of the three STR loci has been constructed successfully. Conclusion: The system of triplexing PCR of three STR loci (D3S1754,D4S1647 and D12S391) are established successfully, is convenient and reliable. The expensivesequencing and imported kits are not necessay in this system. It may be helpful for developing other silver staining or fluorescent labeled multiplex amplification methods.