The Study Of The Effect Of Gengigel Gel In The Treatment Of Plaque-induced Gingivitis

Plaque-induced gingivitis is a chronical inflammatory in the gingiva. Although it was not involved the alveolar process and conjunction epithelial, gingivitis is the earlier appearance of periodontitis and it could develop periodontitis, harming human oral health.Hyaluronic acid is the main component in the Gengigel gel. Hyaluronic acid (hyaluronan) is a glycosaminoglycan with 200-10000 disaccharides and a molecular weight exceeding 10~6 Da. It was found in the extracellular matrix, especially of umbilical cord, synovial fluid, vitreous body and dermis. Hyaluronan has important physiological and cell biological functions and hyaluronan has been assigned various physiological functions in the intercellular matrix, e.g., in water and plasma protein homeostasis. Hyaluronan production increases in proliferating cells, cells recognition, cells locomotion and the polymer may play a role in mitosis. Hyaluronan seems to play an important role during development and differentiation and has other cell regulatory activities.The Objective of the study is to observe the bacteriostatic effects of Gengigel gel on the suspected periodontal pathogens, including P.gingivalis, P.intermedia, F.nucleatum and A.actinomycetemcomitans strains in vitro and the clinical effectsof local application of Gengigel gel (0.8%hyaluronic acid gel) in plaque-induced gingivitis was also evaluated. The bacteriostatic effects of Gengigel gel on some suspected periodontal pathogens in vitro was observed by scrip method. In this split mouth design study 30 volunteers with plaque-induced gingivitis were included. At least two molars and (or) premolars in each quadrant of each one's oral cavity were tested. Each quadrant of the oral cavity gave one of the treatments in local application of Gengigel gel adjunctive to sacling, scaling alone, local application of Gengigel gel and blank control, and defined the group SG, group S, group G and group C correspondingly. Plaque index(PlI), gingival index(GI) and sulcus-fluid-flow-rate(SFFR) were monitored before treatment, 4 days after treatment and 7days after treatment. The results suggested that Gengigel gel had bacteriostatic effects on P. gingivalis, A. actinoraycetemcomitans,F. nucleatum and P. intermedia. When Gengigel gel was diluted threefoldly (HA at 2mg/ml),it had bacteriostatic effects on P.gingivalis, F. nucleatum, A. actinomycetemcomitans and P. intermedia and when sevenfoldly (HA at lmg/ml),it also showed bacteriostatic effects on P. gingivalis and P. intermedia. And we observed that the GI ,P1I and SFFR in group SG decreased significantly faster than that in the group S, (PO.05) and the GI ,P1I and SFFR in group G also decreased significantly faster than that in the group C(P<0.05). From this study we observed that Gengigel gel showed bacteriostatic effects on some suspected periodontal pathogens in vitro and it showed positive effects in the treatment of plaque-induced gingivitis.