Research On Using FRTL-5 Cell In Screening Methods In Vitro Of Environmental Throid Hormone Disruptors

To study the feasibity of using FRTL-5 cell in screening methods in vitro of environmental thyroid hormone disruptors. First, study the cytotoxicity of amitrol, pendimethalin, nitrofen,PCP,ETU,Prodiamine,using MTT. Then using RIA(radioimmunoassay) to test the concentration of TG in culture; using ICC to test the level of TG in FTRL-5 cell and the level of TTF-1 in nuclear; using enzymecellchemistry to test the activity of thyroid peroxidase in FRTL-5 cell.In MTT test, there is distinct cytotoxicity in all dose of amitrol. When the concentration of PCP exceeds 0.0027ng/ul;ETU exceeds 360ng/ul;pendiametnalin exceeds 2500ng/ul;prodiamine exceeds 0.04ng/ul;nitrofen exceeds 100ng/ul,trere is distinct cytotoxicity.In RIA, the concentration of TG in culture is decreased distinctly in medium and high dose of amtrol, ETU, PCP. The concentration of TG in culture is increased distinctly in medium and high dose of pendimethalin, prodiamine. All dose of nitrofen have not distinct effects on the concentration of TG in culture.In ICC test, the concentration of TG in cell is descreased distinctly in high dose of amitrol and pendimethalin. The other environmental chemicals have not obvious effects on the concentration of TG in cell. The level ofTTF-1 in nuclear is obviously decreased in high dose of PCP, pendimethalin and all dose of ETU. The other environmental chemicals have not obvious effects on the level of TTF-1 in nuclear.In enzymecellchemistry test, the activity of TPO is decreased obviously in high dose of amitrol and pendimethalin. The other environmental chemicals have not obvious effects on the activity of TPO in cell.The study obtained expected results and further evidenced the feasibity of using FRTL-5 cell in screening methods in vitro of environmental thyroid hormone disruptors. The obtained results will be the base of future research.