Analysis About The Changes Of Small Intestinal T Lymphocytes In Rats After Scalded

Objective: To explore the distributional law of TCRαβT cells and TCRγδT cells in the epithelium and lamina propria of different small intestines in normal rats and compare with the changes of those in rats after scalded. Clarify the law of immune functional change in the small intestine and provide new thoughts and morphological data to prevent and cure the infection from the intestine after scalded. Methods: Forty male Wistar rats were divided randomly into four groups (n=10), namely control group, postburn 1d(24h) group, postburn 3d(72h) group and postburn 7d group. The control group was not scalded, the three experimental groups were scalded on the backside, inflicted with 30%Ⅲo scald, duodenums, jejunums and ileums from which were isolated on the 1st(24h), 3rd(72h) and 7th day after scalded. Guts from the control group were prepared without scalded. Labeled CD3+T cells and TCRγδT cells using ABC immunohistochemical technique with mouse anti rat CD3 antibody and TCRγδantibody and measured their volume (Vv), numver density (Nv), surface area density (Sv), mean volume (V) and Rsv with the stereological methods. Compared their distribution in the epithelium with that in the lamina propria of different small intestines using statistical software SPSS 10.0 for window. Results:(1) In the duodenum and jejunum of control group, Nv of TCRγδiIELs were larger than that of TCRαβiIELs, while in the ileum, the result was reverse. From duodenum to ileum , Nv of CD3+iIEL and TCRγδiIEL decreased in turn, while Nv of TCRαβiIELs increased. (2) In the small intestine, Nv of TCRαβLPLs were larger than that of TCRγδLPLs. From duodenum to ileum , Nv of CD3+LPLs and TCRαβLPLs increased in turn, while Nv of TCRγδLPLs were stable. (3)In the duodenum and jejunum, Nv of CD3+iIELs and TCRγδiIELs were larger than those of the lamina propria, while in the ileum, the result was reverse. Nv of TCRαβiIELs were smaller than those of the lamina propria in all small intestine. (4) V of TCRγδT cells was larger than that of TCRαβT cells. V of IELs was larger than that of LPLs. (5) IELs were more irregular than LPLs. (6) Nv of CD3+T cells, TCRαβT cells and TCRγδT cells in the small intestine decreased with the time after scalded, except Nv of TCRγδT cells in the ileum which were stable. Volume, shape and distribution of T cells in the small intestine after scalded were same to those of the control group. Conclusion: (1) In the control group, CD3+T cells, TCRαβT cells and TCRγδT cells had regular distributions in the epithelium and lamina propria of small intestine. As a whole, from duodenum to ileum, amounts of T cells decreased in turn, the density of T cells in the epithelium was lower than that of the lamina propria. (2) Amounts of CD3+T cells, TCRαβT cells and TCRγδT cells in all small intestine decreased with the time after scalded, except amounts of TCRγδT cells in the ileum which were stable. (3) Either scalded or unscalded, volumes of TCRγδT cells were larger than those of TCRαβT cells. Volumes of IELs were larger than those of LPLs. (4) Either scalded or unscalded, IELs were more irregular than LPLs. (5) The results indicat that the decrease of T cells after scalded might be related to the development of postburn infections from the intestine.