| Objective: To establish a method for culturing the fracture hematoma cells in vitro and investigate the growth and osteogenesis characteristics of the fracture hematoma cells.Methods: The fracture hematoma cells were cultured in routine and experimental culture medium, observed under the phase-contrast microscope, and osteogenic characteristic was examined by applying alkaline phosphatase(ALP) activity, calcium stains and determination of osteocalcin( OCN) concentration.Results: 1. The rabbit fracture hematoma cells kept active proliferative capacity in vitro.2. The hematoma cells'proliferation speed increased in the conditional medium and showed osteoblastlike structures and functions.3. The ALP and calcium stains of induced cells showed positive reaction; the OCN concentration was higher in the experimental medium than that in the routine medium.Conclusions: 1. Under appropriate conditions, the fracture hematoma cells could be cultured and induced.2. The osteoblastic differentiation of rabbit fracture hematoma cells were improved in the experimental culture medium.3. The cultured cells were osteogenic potential cells. |
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