The Study Of Enrichment And Isolation Of Fetal Cells From Peripheral Maternal Blood And Its Application In Noninvasive Prenatal Diagnosis

Objective: To compare mononuclear cells tabled by CD71 or HbF single-staining method with CD71 and HbF double-staining one. To sort nucleated red blood cells in peripheral blood of pregnant women by flow cytometry using the latter method, and investigate the relationship of frequency of NRBC to gestational age.Methods: Mononuclear cells in umbilical blood and peripheral blood in normal pregnant women and unpregnant women were labled by erythrocyte specific antibody CD71 and fetal specific antibody HbF. Furthermore, NRBC were sorted by flow cytometry.Results: Compared double-staining method with single-staining method, the positive labeling rate of mononuclear cells in all cases was significant different.(p<0.01). Meanwhile, CD71 and HbF double-staining method was successfully used in sorting NRBC in maternal blood by flow cytometry and the purity is(62±2.5)%. Frequency of NRBC increased until 21~24 week's gestation.Conclusion: CD71 and HbF double-staining method could improve NRBC specific lable, sorting purity and make advantage for the next prenatal diagnosis. The optimum time of prenatal diagnosis using fetal NRBC in maternal blood should be at the 21 ~24 week of gestation.