Studies On Affects Of Oxymatrine On Mice Allergic Contact Dermatitis Stimulated By DNFB And It's Immunologic Mechanisms

Objectives: Through analysing Oxymatrine's(OMT) restrain effect of each dosage group on the allergic contact dermatitis(ACD) stimulated by dinitrofluorobenzene (DNFB) and the comparative study of its effects on the quantity of mouse's regulatory T cells(Tr cells) in the peripheral blood. at the same time, to investigate the effect of OMT on mouse lymphocyte proliferation stimulated by con A, probe into the immunological mechanism that OMT treats ACD , is in the hope of offering deeper theory and experiment basis for the thing that treat the anaphylactic disease clinically with OMT.Methods: To build up an ACD mouse model stimulated by DNFB, then takeintraperitoneal injection(IP) by different dosages of OMT , PBS and hydrocortisone(HCT),after that observe the curative effect of OMT by measuring the swelling degree of mouse's auricles.To utilize Carboxyfluorescein diacetate succinimidyl ester(CFDA-SE) dyed and then flow cytometer to examine the fluorescence intensity changes of lymphocytes co- stimulated by polyclonal stimulator Con A and OMT. Whereafter, analyze the efficacy of OMT on mouse lymphocyte proliferation by related software. collect blood In the process of experiment of 1d , 7d , 14d , 21d and 28d via mouse tail vein after giving medicine through IP separately, and then mark the T cells with anti- CD3 ,anti-CD4 , anti-CD25 three-colored immune fluorescence antibody ,and then flow cytometer to examine the ratios of CD4~+CD25~+ T of CD4~+T cells, count the CD4~+CD25~+T cell quantity of each group. The experimental data is dealt with in SPSS 10.0 and CELLQuest software.Results : Comparing with PBS ,OMT possesses the strong effect of restraining the ACD caused by DNFB and depends on its injection dosage. Its restrain effect is equivalent to the HCT of the same dosage, but brings fewer side effects.in vitro experiments prove that OMT has the ability to restrain the proliferation of lymphocyte of mouse depended on its concentration in substrate as it behaves differently in group500, 125 and 31μg/mL, but in 16, 8, 4, 2μg/mL dosage of OMT of each group,itimprove the proliferation of T lymphocytes of mouse's lymph node,while the dependence on its concentration in substrate is not significant, intraperitoneal injection of OMT can improve mouse CD4 +CD25 +T cell quantities in peripheral blood obviously , compare OMT groups with HCT groupfP <0.01), compare OMT groups with PBS group (P <0.01).Conclusions I (l).OMT possesses the obvious effects of restraining the ACD stimulated by DNFB. (2).The effects of OMT on the proliferation of lymphocytes of mice's lymph node T cells presents the two-way function, OMT can obviously improve the quantity of CD4 +CD25 +T cells in the peripheral blood of mouse through intraperitoneal injection, OMT presents the two-way functions on immune system, OMT is a kind of two-way immunoregulator.