Expression Of Survivin And TRAIL In Hemangioma

Hemangioma is a benign tumor of skin and it is one of the most common inborn tumors of infancy. Hemangioma is mostly seen in the head,the face, the neck, and four limbs etc, Hemangioma traditionally includes capillary hemangioma (strawberry hemangioma and port-wine stains) , cavernous hemangioma and racemose hemangioma. The classification based on clinic symptoms and configurations. Unfortunately, the classification can not shows the cells' biologic characteristics of every kind of hemangioma. Based on endothelial characteristics, Mulliken and Glowaki classified the inborn vascular lesions as hemangioma and vascular malformation in 1982. Now, hemangioma's formation and evolution are not clear, with the rapidly development of cell biology and molecular biology, more and more people pay attention to the function of cell apoptosis during the formation and evolution of hemangioma. Many scholar has confirmed that hemangioma result from the lack of apoptosis and the block of apoptosis; it's regression also has great relationship with cell apoptosis. Survivin make Caspase3 be in action directly, it mostly restrain the activation of Caspase3 and Caspase7, blocking endothelium's apoptosis; Survivin also can use P21 to restrain the activation of Caspase3, Survivin can integrate the regulator of cell cycle CDK4 into complex Survivin-CDK.4, hence P21 can be released from the complex and integrate Caspase3 into a new complex. The new complex can restrain the activation of Caspase3 and block endothelium's apoptosis. TNF related apoptosis inducing ligand (TRAIL) or Apo 2 ligand (Apo2L), which is a member of the family of tumor necrosis factors. TRAIL and it's receptor Fas and Caspase8 constitute death inducing signaling complex,DISC, which activateCaspase3. So it can accelerate endothelium's apoptosis. In this study by using immuirmohistochimistry method, we detected the expression of Survivin and TRAIL in 56 samples of hemangiomas and vascular malformations to investigate the the expression of these proteins and the formation and evolution of hemangioma. It will provide new aims for prevent and therapy hemangioma. Materials and methods:The tissue of vascular lesions was obtained from 56 patients who were underwent surgery form January 2000 to December 2002, There were 19 males and 37 femanls. The tissue was divided into two groups: 40 hemangioma (including 26 proliferating hemangiomas and 14 involuting hemangiomas ) ;16 vascular malformations. All the samples should be confirmed by pathology. Every sample was classified by the criterions of Takahashi. With Rabbit Anti-Survivin and Rabbit Anti-TRAIL, we used an immunohistochemical SP method to detect the expression of Survivin and TRAIL. The data we have collected were dealt with soft SPSS10.0, and less than 0.05 are considered significant. Results:1. The positive expression of Survivin was mainly localized in the cytoplasm of endothelium, The Survivin-positive rates in proliferating hemangioma, involuting hemangioma, vascular malforations were 76.9% (20/26),35.7% (5/14) and 25%(4/16) respectively. There was significant difference between the three pathologies (p<0.01). The difference between proliferating hemangioma and involuting hemangioma was significant (p<0.05). The difference between proliferating hemangioma and vascular malforations was also significant (p<0.05). But no significant difference between involuting hemangioma and vascular(p>0.05).2. The positive expression of TRAIL was localized in the cytoplasm and endosmosis of endothelium, The TRAIL-positive rates in proliferating hemangioma, involuting hemangioma, vascular malforations were 23.1%(6/26). 78.6%(11/14) and 0% respectively. There was significant difference between the three pathologies(p<0.05). The difference between involuting hemangioma and proliferating hemangioma was significant (p<0.05). The difference between involuting hemangioma and vascularmalformations was significant (p<0.05). The difference between proliferating hemangioma and vascular malformations was significant (p<0.05).3. The expression of Survivin was correlated with TRAIL in proliferating hemangioma (p<0.05)4. The expression of Survivin was correlated with TRAIL in involuting hemangioma, (p<0.05)Conclusions:1 Overexpression of Survivin in proliferating hemangioma, suggest that Survivin may correlate with the formation of hemangioma.2 In involuting hemangioma, overexpression of TRAIL suggest that TRAIL may have great relationship with involuting hemangioma.3. The expression of TRAIL has a negative correlation with the expression of Survivin in proliferating hemangioma. It may expressed that TRAIL may reastrain the expression of Survivin in proliferating hemangioma and restrain the proliferation of hemangioma.4. The expression of Survivin has a negative correlation with the expression of TRAIL in involuting hemangioma. It may expressed that Survivin may reastrain the expression of TRAIL in involuting hemangioma and restrain the involution of hemangioma.5. The difference that Survivin and TRAIL expressed in proliferating hemangioma and involuting hemangioma was of great benefit to the research of hemangioma, the treament of hemangioma and the prognosis of hemangioma.