Effect Of Coumarin On Lymphostatic Cerebroedema

ObjectiveThe lymph flows in the brain, which is very important for the normal role of brain. All causes that can occlude lymphatic drainage of the brain in rats will lead to the animals suffering from lymphostatic cerebroedema and the normal function of its brain is disturbed. The research results from the animals' experiment found that it was obviously altered in the configuration and function of the brain and the stress of the cerebrospinal fluid. But the treatment of this disease is not reported in home.So finding out a kind of medicine to cure this disease has been brought into focus.Coumarin is a kind of medicine which is effective to the lymphedema. So we use Coumarin to treat Lymphostatic cerebroedema.And we hope to provide some information for the clinical treatment of LC from our experiment.Materials and methodsThe rats were divided into normal rats and model rats and the Coumarin group. Ligate all the lymphatic vessel in the neck and make the model of Lymphostatic cerebroedema.To the Coumarin group rats Coumarin were continuously injected intraperitoneally from the first day of operation to 21d after operation. Then the Morris water maze task was performed among all groups in 7d , 14d , 21d after the operation. The EEG and Blood pressure was recorded in model group rats and the medicine group rats in the same time. The hippocampus tissues of their brains were studied in transmission electron microscopic level. And the synaptophysinimmuno label ing density was estimated in freezed hippocampus of rats.In 21d after the operation all items were measured in control group rats.Finally make analyzed of all data.Results1. Morris water maze ethologicai test:In the 7d after the operation the medicine group had no significant difference in the escape latency with the model group,while had longer escape latency than the control group and had significant difference with them;In the I4d and 21d after the operation the medicine group had significantly shorter escape latency than the model group and had significant difference compared with them(P <0.05); In the 14d after the operation the medicine group had significantly longer escape latency than the control group and the medicine group and had significant difference compared with them(P <0.05). In the 2Id after the operation the medicine group had no significant difference compared with the control group(P >0.05).In the Id. I4d> 21 d after the operation the model group had significantly longer escape latency than the control group and had significant difference compared with them(P<0.05).2. Blood pressure(BP)The model group had higher blood pressure than the control group in 7d after the operation,but had no significant difference compared with the control group(P>0.05). The model group had significantly higher blood pressure than the control group in 14d and 21d after the operation and had significant difference compared with the control group (P<0.05).The medcine group had higher blood pressure than the control group in 7d and 2Id after the operation and the medicine group and had no significant differencecompared with the control group(P>0.05). The medcine group had significantly higher blood pressure than the control group in 14d after the operation and the medicine group and had significant difference compared with the control group(P <0.05). The medcine group had significant difference in 14d and 2Id after the operation compared with the model group.3. EEGThe control rats have the medium wave amplitude of EEG. The model group had no significantly altered in wave amplitude of EEG in 7d after the operation. The model rats had significantly lowered in wave amplitude of EEG in 14d and 2Id after the operation. The medcine group had significantly higher wave amplitude of EEG than the model group in 14d and 2Id after the operation, and in 2Id after the operation had as high wave amplitude as the control rats.4.Ultrastructural alteration of hippocampus in the model group and the medicine groupThe brain tissue was obviously loose and the perivasular spaces around the capillaries were full of tissue fluid and found the edematous fluid in the brain tissue of model rats in 14d and 2 Id after the operation. The mitochondrin were swelling and mitochondrial cristae were fragmented or disappeared,some mitochondrina showed vacuolar degeneration.In 14d after the operation the hippocampus tissue of medicine group fluid was still exist in perivasular spaces around the capillaries,but reduced a lot. In 2Id after the operation the brain tissue of medicine group perivasular spaces around the capillaries was minished and was close to normal.5. the synaptophysin immunolabeling density in freezed hippocampus of ratsThe synaptophysin immunolabeling density in freezed hippocampus of model rats in I4d after the operation had significant difference compared with the controlled group and the medicine group(P<0.05) .In 14d after the operation the synaptophysin immunolabeling density in freezed hippocampus of medicine group had significant difference compared with the controlled group; In 2Id after the operation the synaptophysin immunolabeling density in freezed hippocampus of medicine group had no significant difference compared with the controlled group.ConclusionThe model of Lymphostatic cerebroedema which was produced by ligating all the lymphatic vessels in the neck is credible model for studying related experiment. Lymphostatic cerebroedema has obviously influence in the function of brain,which can exert an influence on the configuration of the model rats' brain and the spatial reference memory and on the EEG and blood pressure of the model animals.Coumarin can reduce the Lymphostatic cerebroedema.