| The ultimate destination of tooth regeneration is to replace the damaged tooth with regenerated teeth holding normal morphology and physiological function through integrating odontogenic tissues or cells into human body. In this essay, we inspected the odontogenic behavior of human bone marrow stromal stem cells (BMSSC) in recombinant tooth germs to find a appropriate kind of cell or regenerative model for the tissue engineering of tooth regeneration. In our research, the inductive conditions and surface markers were employed to investigate the biological characteristics of the BMSSC isolated and cultured ubder our lab conditions; through engrafting intact tooth germs from abortive human embryos and chimera tooth germs into renal capsule of immuno-compromised mice, we substantiate the developmental prosess of human tooth gerns out of in situ and feasibility of forming tooth from chimera tooth germs ;the odontogenic behavior of BMSSC in several kinds of regenerative models-inductive or non-inductive and reaggregated or recombinant models constructed by integrating human BMSSC into mouse tooth germs, were inspected and analyzed. Sum up above results, we conclude that the recombinant model is more fit to tooth regeneration than reaggregated model; the chimera tooth germs containing mouse tooth germs and human BMSSC can develop into dental structures on host renal capsule, as well as the intact human tooth germs, and human BMSSC participate in the construction of these dental structures.
|
PDF Full Text Request