Study On Changes Of Cellular Immunity Caused By Infusion Of Rhesus Mesenchymal Stem Cells Intravenously Or Intra-bone Marrow

Many experiments in vitro testified that bone marrow mesenchymal stem cells(BM-MSCs) could suppress T-lymphocytes proliferation induced by allogeneic cells. It is of much clinical importance to deeply study MSCs' immunological characteristics because T-lymphocyte immunity is involved in many kinds of disease such as graft versus host disease(GVHD) after allogeneic hematopoietic stem cell transplantation (Allo-HSCT).To study the changes of cellular immunity caused by infusion of MSCs intravenously or intra-bone marrow (IV or IBM). Firstly, we successfully isolated and cultured BM-MSCs, then evaluated the immunomodulatory effects caused by MSCs infusion(IV or IBM), including peripheral blood counts, mixed lymphocyte reaction (MLR), fluctuation of lymphocytic subgroup, skin explant culture and TUNEL test.No acute toxicities or GVHD were observed in either IV or IBM group. No obvious changes of peripheral blood counts appeared. Within 14 days post MSCs infusion, relative reaction(RR) of MLR decreased in both IV and IBM group, and the decreasing degree was related to the amount of MSCs. The MSCs administered intra-bone marrow was more effective in inhibition of T-lymphocytes immunity than those administered intravenously.After MSCs infusion, CD3~+ CD3~+CD4~+ and CD3~+CD8~+ T-lymphocytes decreased in two recipients who were administered the largest number of MSCs (2.0 × 106/kg), and restored within 30 days. But no obvious changes were found in other rhesus monkeys.Within 20 days post MSCs infusion, the skin explants' pathological lesion of either the donor or the third monkey lessened and apoptosis cells in TUNEL test declined in both IV and IBM group, which indicated that MSCs infusion could induce...