| Objective To evaluate the value of detecting KatG genemutation in Mycobacterium tuberculosis by polymerase chain reaction(PCR) and single-strand conformation polymorphism (SSCP)analysis .Methods 20 drug-susceptible M. tuberculosis and 30isoniazid-resistant M. tuberculosis were evaluated by PCR-SSCP ,with H37Rv reference as control group .Results 20 drug-susceptible clinical isolates and M .tuberculosis H37Rv strain displayed identical SSCP patterns . Part ofisoniazid-resistant clinical isolates shows a different SSCP patternsfrom M . tuberculosis H37Rv strain , and a few isoniazid-resistantclinical isolates displayed the same patterns . Compared with drugsensitive test , the positive rate to detect KatG gene by PCR-SSCP was65.7% , and specificity was 95% .Conclusion The changes of KatG gene are associated withisoniazid resistance . PCR-SSCP technique is a simple and reliablemethod of detecting KatG gene mutation , and it will be useful inrapid detecting the isoniazid-resistant M . tuberculosis . |
