Clinical And Experimental Study Of Tongluo Yanggan Mixture Treating Earlier-cirrhosis

Purpose : Observe the curative effect and the advantage caused by Tongluo Yanggan mixture(TLYG) in terms of anti-hepatic earlier-cirrhosis, and reveal the mechanism of anti-hepatic fibrosis.Method : Clinic study: 60 patients of earlier-cirrhosis after hepatitis B or hepatitis C whose syndrome was blood stasis were therapied with TLYG There is no control class. The results was compared with before and after the therapy. Experimental research: The rats in which hepatic fibrosis model was induced by carbon tetrachloride (CCL4) were divided into three groups. Except the model and control groups, the prevention and therapy groups all received TLYG in different period for 8 weeks by poured in. After all rats were put into death, tested the indexes of hepatic function and the contents of HA,LN,PCIII in fibrosis and SOD, MDA in peroxidation in serum. The histopathology of hepatic tissue was observed by histological examination and Masson's dye which were performed in hepatic tissue, and immunohistochemical assay which the expression of Desmin was measured by, so we can comprehend the changes of denaturation of hepatic cells, hyperplasia of collagen and the activation of hepatic stellate cells in terms of semi-quantity in all groups.Results :After counted with statistics, there were vary amendments in clinic symptoms, hepatic function, the indexes of fibrosis and the show of liver and spleen in Doppler of 60 patients, and the discrepancies compared between before and after treatment were obvious (P<0.05 or P<0.01). The indexes in serum and the semi-quantities of denaturation of hepatic cell, hyperplasia of collagen and the activation of HSG in hepatic tissue of treatment groups had better amendments than those in model group, and the discrepancies compared among them are significant. The effect in therapy group was better than that in prevention group.Conclusion TLYG has accurate curative effects, including the protection of hepatic cells, the depression of collagen's hyperplasia and the antagonism of hepatic stellate cell's activation. So it can ameliorate the symptoms and the indexes tested in lab of patients.