| Background & Objective: Gastric carcinoma remains one of the most common carcinomas,lots of research have been shown that the develping of gastric carcinoma undergo a long stage- gastric precancerous lesion. People have not know clearly the mechanism of gastric carcinoma by now. With the develping of molecular biology , people have know that from normal gastric mucosa to gastric carcinoma often involve many genes and many biology processes. It will help to explain the mechanism of gastric carcinoma and the early diagnosing gastric carcinoma,if the relationship between the different tumor genes and the gastric precancerous lesion could be found. Survivin gene is a novel inhibitor gene of apoptosis and is a new member of inhibitor of apoptosis protein .Survivin only expresses in the tissue of embryo and tomor.It can not be detected in the normal tissue. In the physiological condition,Survivin can clear away the old and abnomal cell by the apoptosis of cell . In the early stage,many factors which lead to inhibite cell apoptosis were benefit of proliferation of cells and abnomal genes accumulation and the develping of gastric carcinoma. Tumor suppressor genes,which inhabit cells growth and proliferation, have a potential function in preventing the occurrence and growth of tumors. Phosphatase and tensin homologue deleted on chromosometen (PTEN) , the first identified suppressor gene that have phosphatase activity,has an important role in signals condition . p53 gene have highly correlated with human tumors . p53 protein has a function in regulating the proliferation cells of cellcycle. Ki-67 is a nuclear which connected with proliferation cells. Many tumors and precancerous lesions can be detected the expression of ki-67.It might play an important role in regulating process of tumor invasion and metastasis. The present study was designed to investigate the protein expression of Survivin, PTEN, p53, ki-67 and their relationships in the gastric precancerous lesion and gastric carcinoma,and to assess the possibility for the early diagnosing gastric carcinoma with tumor gene Methods: The immunohistochemical SP method was applied to examine the expression of Survivin, PTEN, p53 and ki-67 in normal gastric mucosa ,chronic superficial gastritis, atrophic gastritis without intestinal metaplasia,atrophic gastritis with intestinal metaplasia, mild dysplasia moderate dysplasia, severe dysplasia and gastric carcinoma. The data were processed with SPSS 12.0. Results: The positive expression rates of Surviving , PTEN ,p53 and ki-67 were 0%,100%,0%,0%,respectively ,in normal gastric mucosa. The positive expression rates of theirs were 0%,100%,0%,18%,respectively, in chronic superficial gastritis.The positive rates of theirs were 0%,93%,0%,33%, respectively, in atrophic gastritis without intestinal metaplasia. The positive expression rates of theirs were 0%,91%,0%,39% respectively, in atrophic gastritis with intestinal metaplasia. The positive rates of theirs were 7%,77%,7%,54% , respectively, in mild dysplasia. The positive rates of theirs were 20%,70%,30%,60% ,respectively, in moderate dysplasia. The positive rates of theirs were 50%,63%,63%,75%,respectively, in severe dysplasia. The positive rates of theirs were 56%,60%,68%,88% ,respectively,in gastric carcinoma The positive expression rate of Survivin was observed in dysplasia and carcinoma.No significant difference was observed for Survivin expression level between severe dysplasia and carcinoma(p>0.05). The positive expression rate of PTEN was observed and was down regulated in the in gastric carcinoma and precancerous lesion . No significant difference was observed for PTEN expression level between dysplasia and carcinoma(p>0.05).No significant difference was observed for p53 expression level between severe dysplasia and carcinoma(p>0.05). The positive rate of ki-67 was observed and was up regulated in the in gastric carcinoma and precancerous lesion . No significant difference was observed for PTEN expression level between dysplasia and c... |
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