| Liuwei Dihuang Decoction is a famous represen prescription, it is quoted from 《xiao 'eryaozhengzhijue》 , It is composed of shudi, shanzhuyu,shanyao, fuling, zexie, and danpi.Pharmacology make clear that the research expresses Liuwei Dihuang Decoction having extensive pharmacological action. Recently Liuwei Dihuang Decoction is paid close attention to immunopharmacology. We also proceeded a series of research to Liuwei Dihuang Decoction' s pharmacological action,it proved that Liuwei Dihuang Decoction has the obvious pharmacological action, we find a phenomenon that it can alleviate the weight of thymus when Liuwei Dihuang Decoction anti glucocorticoid indution . because atrophy induced of glucocorticosteroid have something to do with the lymphocyte apoptsis induced of glucocorticosteroid. It hinting that Liuwei Dihuang Decoction may have some functions on anting Hydrocortisone cause thymus and spleen apoptosis.Objective: This experiment aim at with the Hydrocortisone induce the lymphoid cell apoptosis for model, study Liuwei Dihuang Decoction to the in restraint of function of the lymphoid cell apoptosis and to related influence that gene expresses, to announce to public should that has new immunity pharmacological action.Methods: A number of methods have now been developed to study apoptosis in cell populations. We focus on three key apoptotic events in the cell:1. According to pathologic changes that appearance learn, the appearance learns can for the experiment proof that cell apoptosis offering is obviously;2. Apoptosis and cell mediated cytotoxicity are characterized by cleavage of the genomic DNA into discrete fragments prior to membranedisintegration. Because DNA cleavage is a hallmark for apoptosis, assays which measure prelytic DNA fragmentation are especially attractive for the determination of apoptotic cell death. The DNA fragments may be assayed in either of two ways:_As "ladders" (with the 180 bp multiples as "rungs" of the ladder) derived from populations of cells, e. g., with the Apoptotic DNA Ladder Kit (described on page 11 of this guide). Thus, in the following sections, we will describe details of each of these apoptosis assays.3. the cytology method,.. then flow cytometry , observes to keep the square diagram, see whether at two times cell peaks( Ap peak ) to appear two second times cell peaks ( Ap peak ) come to judge whether to exsit cell apoptosis or not, and can pass the Ap peaks high to come to judge the cell apoptosis rate.Results:1. It can be discovered that Liuwei Dihuang Decoction learns with the morphology of the lymphoid cell of spleen and the thymus gland in the research: Liuwei Dihuang Decoction let up the anti- function with the spleen and the thymus gland. And Liuwei Dihuang Decoction let up with the spleen index at the small rat in the thymus gland the better than IL-2, and Liuwei Dihuang Decoction let up with spleen index number at the small rat have something to do with measuring, the high dose surpasses the low dose, bottom It can the find out the normal T cell in Electron microscope, and the dying is well-distributed, and can chick out the apoptosis cell , Other each too can find the inequality cell of the apoptosis cell in Electron microscope.2. It can be discovered that Liuwei Dihuang Decoction learns with the biochemistry of the lymphoid cell of spleen and the thymus gland in the research: DNA "Ladder" can be find in every group. And Liuwei Dihuang Decoction take the color become shallow, combine to report the deal effect relation.3. It can be discovered that Liuwei Dihuang Decoction learns with the fixed quantity of the lymphoid cell of spleen and the thymus gland in the research: Liuwei Dihuang Decoction can check out the AP, It is notable that the rate of apoptosis' s difference in that six group (P<0.01).Conclusion:1l.The Electron microscope display the change in the apoptosis cell, gel electrophoresis display the DNA "Ladder" , the AP disply in the FCM; it express that the model is success.2. Liuwei Dihuang Decoction can anti glucocorticoid indution thymus and spleen atrophica, it may be that Liuwei Dihuang Decoction anti lymphocyte abnormily apoptosis.3. The GC expresses six the DNA ladder forms become shallow, combine to report measures dependence;The FCM examines the Liuwei Dihuang Decoction' s AP diminish;The result expresses Liuwei Dihuang Decoction... |
