Study On The Retroviral Vector PLXSN-hBDNF Modified Neural Stem Cells And Its

Spinal cord injury (SCI) is commonly encountered in clinical.The number of patients with SCI is significantly increasing every day. Traumatic spinal cord injury can result in severe damage, leading to paraplegia, or worse. Many strategies, including surgical, pharmacolo gical, have been used in attempts to develop new therapies that will allow patients to regain the use of their paralyzed limbs. One new strategy is neural stem cell transplantation into the damaged spinal cord. The cell transplanted can replacement of damaged interneurons within a structure, promote the functional reconstruction of neuronal circuits, and recovery the function of spinal cord. The present research is, through biomolecular technique, to constitute a retroviral vector integrated human BDNF, then to infect the neural stem cells in vitro, and induce its differentiation, Which provide new evidence to the clinical application of the spinal NSCs.In Part 1 we isolated clone cells from El4 fetus mice's spinal through non-serum cell culture. Immunohistochemistry was used to identify the cell type, and then Spinal cord cells were induced differentiation with several different extracellular factors.The result we found it that:1. The spinal cord derived NSCs are capable of proliferation, repeated subculture (self-replicating capacity), expressing Nestin antibody and dif...