The Primary Research On The Retinal Development Of The Mice With Retinal Degeneration

The animal models with the human's inherited diseases are vital to the mapping of the genes, the functions of the genes, and the research for the remedy for the diseases. Nowadays, there are some kinds of animal models with different inherited diseases. The Jackson Lab is the one of the labs that has a lot of kinds of models, from which we can get at least 16 kinds of mouse models with spontaneous retinal degeneration (rd). The mice are useful for studying the rd. Our country had no the mouse models with inherited diseases. In recent study, we had found some kinds of models with inherited retinal diseases, one of which is a kind of mice with retinal degeneration fast (rdf). In this article, the development of the rdf mice were studied to make a preparation for establishing the strain: 1, to establish the inbreeding strain of the KUNMING rdf mice and to transfer the involved gene of the KUNMING rdf mice into the C57BL/6 mice by homologous transformation; 2, the morphologic development of the rdf retina; 3, the development of the cGMP content in retina of the rdf mice; 4, the development of the ERG of the retina; 5, in according with the need for study and work, to compare the two different visual electrophysiology systems, GUOTE (GT-2000NV, made in China) and RETIscan (made in Germany).Methods and material:To establish the inbreeding strain of the KUNMING rdf mice and to transfer the involved gene of the KUNMING rdf mice into the C57BL/6 mice by homologous transformation; to observe the retinal morphology of the rdf mice from the day 10 after impregnation, that is E10, to P56 with the paraffin embed and HE stain; to observe the ultrastruction of the rdf retina from the 1^st week postnatal to the 8^th week postnatal with the transmission electromicroscope; to measure the cGMP content in the rdf retinas from P8 to P20 with radiate competitive immune test; to measure the ERG of the rdf mice from the 2^nd week postnatal to the 8^th week postnatal; to record the standard and multifocal visual electrophysiology of normal people with GT-2000NV and RETIscan systems to compare the data and to get the idea whether the data recorded with GT-2000NV system were reliable.Result1, now the inbreeding strain has been reached to the F9 generation, and the homologous transformation has been reached to the F8 generation.2, the morphology of the rdf retinas with developmentThrough the HE stain, it can be got that the development of the retinas from E10 to P10 has no instinct difference between rdf and wild mice. But at P14, the number of layer in the outer nuclear layer of the rdf mice is half of the wild mice. Consequently, since P21 the outer nuclear layer, as well as the outer plexiform, photoreceptor layer can not be distinguished from microscope.3, the change of the cGMP content of the rdf micethe result was that the cGMP content of the rdf mice were steady from P8 toP16, and that at P20 ,when the outer nuclear layer can not be distinguished, the content of the rdf mice declined distinctly. The differentiation of the cGMP between the rdf and wild mice could be found at P8 and P20.4, the ERG of the rdf mice with developmentAt first the apparatus used was be tested. Two sets of electrophysiological apparatuses, the GT-2000NV (made in China) and the RETIscan (made in Germany) were compared. Consequently, we could reach to a conclusion that there was the relativity between the two sets. And then the ERGs of the rdf and wild mice were tested with the GT-2000NV set. The consequence was that the ERG of rdf mice had no waves since P14, that is the extinguished ERG. On the contrary, the immature waves could be got from the wild mice at P14, when the photo ERGs had appeared and the scoptic ERGs were still immature. The ERG waves of the wild mice reached to mature at P21.5, the most data recorded with GT-2000NV and RETIscan systems were significant different in statistics, but had significant relation.ConclussionInhereted animal model is useful for investigating the involved gene, pathology, therapy and so on. So once the model is concerned, we should preserve the model and keep it from generation to generation in order to study. We have been establishing the inbreeding strain, and homologously transformating the rdf gene into C57BL/6 mice, so that the involved gene could be mapping if the rdf was not the consequence of known genes.The inheritance mode of rdf mice was euchromosome recessive inherit, and the ERG of the rdf mice couldn't be recorded from P14. During development, the retinal degeneration happened from P10. At P21, the outer nuclear layer and outer plexiform layer completedly disappeared. All above were similar with rd1 mice, but...