Experimental Study On RhBMP-2-induced Cartilage Regeneration In Tracheal Grafts With Muscular Wrapping

Tracheal transplantation is one of the important anticipated events in tracheal surgery, regarded as an ideal technique for reconstruction of tracheal defect after extensive resection. The research on tracheal transplantation has progressed slowly in the past years because of the subsequent severe stenosis and obliteration of the tracheal grafts. The major causes consist of ischemia and rejection of tracheal grafts, which result in cartilaginous necrosis, chondrolysis and resorption. Although different processes of airway collapse have been found in tracheal grafts treated by different methods, few suitable methods can be applied to fundamentally solve the problem essentially up to date. So it's necessary to find a new method to prevent the tracheal grafts from the stenosis. Bone morphogenetic proteins (rhBMP-2s) are a group of proteins which can induce new cartilage and/or bone with muscular wrapping, and in this study rhBMP-2 was used to solve the problem.Objective: Aim to induce cartilage regeneration in tracheal grafts wrapped with muscle by utilizing the osteogenesis character of rhBMP-2, and ultimately aim to form a new elastic stent for tracheal grafts to protect the airway from collapse.Methods: Canine tracheal auto- and allo- grafts models were created to observe the correlated factors of stenosis, and then rhBMP-2 was implanted into the canine...