Establishment And Characterization Of Human Osteosarcoma Cell Sub-clones With Different Potential Of Metastasis

Osteosarcoma is the most common malignant bone tumor in adolescents and young adults,which is twenty percent of all bonetumors.although with the development of surgery and screennage technology and achievement of therapeutic alliance of chemotherapy and immunotherapy,the therapy of osteosarcoma has taken a great deal of achievement,the metastasis of the tumor is a question not to be dealt completetly.There are still 40% to 50% patients who die of the recurrence or metastasis,metastasis is still the main question effectting the therapy. Only to understand the mechanisms and the specificities of metastasis ,can we find the effective therapies to overcome the osteosarcoma.The latest reserches showthat metastasis of tumor consists of a serial of steps .including invasion to the surrounding tissue ,move along the blood vessel and lymph vessel proliferation at the target organs and etc. Although some molecules has been found and identified, the complex mechanisms which controls the metastasis are still a secret. To compare with the level of expression of the genes, it is the important that getting the two tumor sub-clones with the same background of the gene.We get the different mono-cell clones by the limited dilution method from the human osteosarcoma cell line sosp-9607 .After preliminary screening through in vitro invasion assay and in vivo selection, we select sosp-9607H9 as the low metastasis potential cell sub-clone and sosp-9607E10 as the high metastasis potential cell sub-clone . After that ,we continued to study their different biological characters by cytometry ,chromosomenumber ,histochemistry, soft agar cloning technique ,PCR,and metstasis in nude mice...