Experimental Study Of Hyperoxia Solution On Fatigue Induced By Exercise And Its Mechanism

Fatigue, a physiological or pathological syndrome, occurs when one works for a long time or has too heavy work and can lead to low motor performance temporarily.Long time or heavy work will make carbohydrate and oxygen consume rapidly, thus leads to the turbulence of the balance of inner environment, which was characterized by the increase of lactic acid. The factors of fatigue from the central nervous system to the peripheral nervous system form a chain to control muscle shrinkage. The breakage may result in fatigue.A number of studies have been carried on anti-fatigue including enhancing resistance and supplying nutrition etc. Chinese traditional medicine and acupuncture also have some curative effect. The basic principle is to ease fatigue factors.We investigate the effect of hyperoxia solution on anti-fatigue function and explore the possible mechanisms, hoping to offer a new approach of anti-fatigue.PART ONE: An experimental study of hyperoxia solution on fatigue and its mechanism in mice induced by exercise STUDY ONE: Hyperoxia solution taken orally on fatigue and itsmechanism in mice induced by exerciseAIM: To investigate the anti-fatigue effects and mechanisms of hyperoxia solution (HO) taken orally on mice induced by exercise. METHODS: The 120 mice were divided randomly into two groups: control group(C group), hyperoxia solution group(HO group).Then each group was divided into three subgroups by dose, which was infused with balanced salt solution or HO at 0.015ml, 0.025ml and 0.05ml per gram, respectively. After the mice were infused intragastricly, the contents of muscle glycogen, lactic acid and malondialdehyde, the general ability of antioxidation and the energy of adenosine triphosphate enzyme were determined instantly and after swimming 30 minute while they were observed about common state. RESULTS: HO group swam more actively and sank fewer than C group (P<0.05). The contents of lactic acid of HO group were smaller significantly than those of C group (P<0.05). The content of muscle glucogen of HO group was bigger distinctly than those of C group (P<0.05). The activity of adenosine triphosphate enzyme of HO group was increased more than those of C group (P<0.05). The content of malondialdehyde of HO group were smaller significantly than those of C group (P<0.05). The general ability of antioxidation of HO group was increased more than those of C group (P<0.05). CONCLUSION: HO has anti-fatigue effect.STUDY TWO: Hyperoxia solution injected on fatigue in mice induced by exerciseAIM: To explore the antifatigue effect of hyperoxia solution (HO) injected and its mechanism in mice induced by exercise. METHODS: The 120 mice were divided randomly into two groups: control group, hyperoxia solution group. Then each group was divided into three subgroups by dose, which were injected with balanced salt solution orHO at 0.1ml, 0.15ml and 0.2 ml per gram, respectively. After the mice were injected intragastricly, the contents of lactic acid, muscle glycogen and malondialdehyde, the general ability of antioxidation and the activity of adenosine triphosphate enzyme were determined instantly and after swimming 30 minute while they were observed about common state. RESULTS: HO group swam more actively and sank fewer than C group (P<0.05). The contents of lactic acid of HO group were smaller significantly than those of C group (P<0.05). The content of muscle glucogen of HO group was bigger distinctly than those of C group (P<0.05). The activity of adenosine triphosphate enzyme of HO group increased more than those of C group (P<0.05). The content of malondialdehyde of HO group were smaller significantly than those of C group (P<0.05). The general ability of antioxidation of HO group was increased more than those of C group (P<0.05). CONCLUSION: HO has anti-fatigue effect. PART TWO: An experimental study of hyperoxia solution taken orally on fatigue in volunteers induced by exerciseAIM: To explore the antifatigue effect of hyperoxia solution (HO) taken orally and its mechanism in volunteers induced by exercise. METHODS: The 60 volunteers were divided randomly into two groups: control group, hyperoxia solution group. Then each group takes balanced salt solution or HO. The heart rate, the contents of lactic acid and malondialdehyde, the general ability of antioxidation and the energy of adenosine triphosphate enzyme were determined instantly and after running 3km.RESULTS: The heart rates of HO group were significantly lower than those of C group (P<0.05). The contents of lactic acid of HO group were smaller significantly than those of C group (P<0.05). The activity of adenosine triphosphate enzyme of HO group increased more than those of C group (PO.05). The content of malondialdehyde of HO group were significantly lower...